Zou Xin, Huang Wenya, Lu Fuer, Fang Ke, Wang Dingkun, Zhao Shuyong, Jia Jiming, Xu Lijun, Wang Kaifu, Wang Nan, Dong Hui
Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.
Shijiazhuang YiLing Pharmaceutical Co., Ltd., Shijiazhuang, 050035, People's Republic of China.
BMC Complement Altern Med. 2017 Mar 23;17(1):165. doi: 10.1186/s12906-017-1648-9.
Jiao-Tai-Wan (JTW), composed of Rhizome Coptidis and Cortex Cinnamomi, is a classical traditional Chinese prescription for treating insomnia. Several in vivo studies have concluded that JTW could exert its therapeutical effect in insomnia rats. However, the specific mechanism is still unclear. The present study aimed to explore the effect of JTW on sleep in obesity-resistant (OR) rats with chronic partial sleep deprivation (PSD) and to clarify its possible mechanism.
JTW was prepared and the main components contained in the granules were identified by 3D-High Performance Liquid Chromatography (3D-HPLC) assay. The Male Sprague-Dawley (SD) rats underwent 4 h PSD by environmental noise and the treatment with low and high doses of JTW orally for 4 weeks, respectively. Then sleep structure was analyzed by electroencephalographic (EEG). Inflammation markers including high-sensitivity C reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were examined in the rat plasma. Meanwhile, metabolic parameters as body weight increase rate, fasting plasma glucose (FPG), fasting insulin (FINS) levels and insulin resistance index (HOMA-IR) were measured. The expressions of clock gene cryptochromes (Cry1 and Cry2) and inflammation gene nuclear factor-κB (NF-κB) in peripheral blood monocyte cells (PBMC) were also determined.
The result showed that the administration of JTW significantly increased total sleep time and total slow wave sleep (SWS) time in OR rats with PSD. Furthermore, the treatment with JTW reversed the increase in the markers of systemic inflammation and insulin resistance caused by sleep loss. These changes were also associated with the up-regulation of Cry1 mRNA and Cry 2 mRNA and the down-regulation of NF-κB mRNA expression in PBMC.
This study suggests that JTW has the beneficial effects of improving sleep, inflammation and insulin sensitivity. The mechanism appears to be related to the modulation of circadian clock and inflammation genes expressions in PBMC.
交泰丸由黄连和肉桂组成，是治疗失眠的经典中药方剂。多项体内研究表明，交泰丸对失眠大鼠具有治疗作用。然而，其具体机制仍不清楚。本研究旨在探讨交泰丸对慢性部分睡眠剥夺（PSD）的肥胖抵抗（OR）大鼠睡眠的影响，并阐明其可能的机制。
制备交泰丸，并通过三维高效液相色谱（3D-HPLC）分析确定颗粒中所含的主要成分。雄性Sprague-Dawley（SD）大鼠通过环境噪声进行4小时的PSD，并分别口服低剂量和高剂量的交泰丸4周。然后通过脑电图（EEG）分析睡眠结构。检测大鼠血浆中包括高敏C反应蛋白（hs-CRP）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）水平在内的炎症标志物。同时，测量体重增加率、空腹血糖（FPG）、空腹胰岛素（FINS）水平和胰岛素抵抗指数（HOMA-IR）等代谢参数。还测定外周血单核细胞（PBMC）中生物钟基因隐花色素（Cry1和Cry2）和炎症基因核因子-κB（NF-κB）的表达。
结果表明，交泰丸给药显著增加了PSD的OR大鼠的总睡眠时间和总慢波睡眠（SWS）时间。此外，交泰丸治疗逆转了睡眠剥夺引起的全身炎症和胰岛素抵抗标志物的增加。这些变化还与PBMC中Cry1 mRNA和Cry 2 mRNA的上调以及NF-κB mRNA表达的下调有关。
本研究表明，交泰丸具有改善睡眠、炎症和胰岛素敏感性的有益作用。其机制似乎与PBMC中昼夜节律钟和炎症基因表达的调节有关。
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