小鼠肾素-1和肾素-2基因座的紧密物理连锁。

Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, Buffalo, NY 14263.

Nucleic Acids Res. 1988 Mar 25;16(5):2111-26. doi: 10.1093/nar/16.5.2111.

In addition to the Ren-1 gene common to all mice, some inbred strains carry a second copy of the renin structural gene, Ren-2. These two loci are tightly linked genetically on mouse chromosome one. We have used pulsed field gel electrophoresis (PFGE) to study the physical arrangement of the two renin genes in the inbred strain DBA/2. PFGE mapping permitted the construction of a restriction map of the Ren loci spanning roughly 120 Kb. The results indicate that the genes are transcribed in the same relative direction, that Ren-2 lies upstream relative to Ren-1, and that the respective coding sequences are separated by approximately 20 Kb.

除了所有小鼠共有的Ren - 1基因外，一些近交系还携带肾素结构基因Ren - 2的第二个拷贝。这两个基因座在小鼠1号染色体上紧密连锁。我们利用脉冲场凝胶电泳（PFGE）研究了近交系DBA/2中两个肾素基因的物理排列。PFGE图谱绘制允许构建跨越约120 Kb的Ren基因座限制图谱。结果表明，这些基因以相同的相对方向转录，Ren - 2相对于Ren - 1位于上游，并且各自的编码序列相隔约20 Kb。