Tan Liming, Zhang Yuhong, Jiang Yongqing, Li Hua, Chen Juanjuan, Ming Feng, Wang Waimei, Yu Jianlin, Zeng Tingting, Tian Yongjian, Wu Yang
Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
2010 Session, The Second Clinical Medical College of Nanchang University Nanchang 330006, P. R. China.
Am J Clin Exp Immunol. 2017 Feb 15;6(2):21-26. eCollection 2017.
The project is aimed to detect anti-mitotic spindle apparatus antibody (MSA) and anti-centromere antibody (ACA) and explore the clinical value for the diagnosis of small cell lung cancer (SCLC), providing clinical evidence for molecular studies of SCLC.
93 SCLC patients, 208 patients with other cancers and 50 healthy controls were enrolled in this study. MSA antibodies were detected by enzyme linked immunosorbent assay (ELISA). MSA, ACA and anti nuclear antibodies (ANA) were examined by indirect immuno-fluorescence (IIF). And the results were retrospectively analyzed.
① the positivity for MSA and ACA by IIF assay was respectively 36.56% and 30.11% in SCLC group, higher than in other tumor groups (P<0.01), ② in correlative analysis, the RR (Relative Ratio) value between MSA and SCLC was as high as 12.93, 12.74, and the RR value of ACA and ANA with SCLC was respectively 4.31 and 3.48. ③ the area under ROC (Receiver operating characteristic) curve (AUC) of MSA detection for SCLC was 0.778, with medium diagnostic value.
MSA and ACA might serve as a new marker for SCLC because of its high detection rate. These two markers may participate in the occurrence and development of SCLC, resulting from the highly strong risk. So, the study have some application value for early detection, clinical diagnosis and potential treatments of SCLC.
本项目旨在检测抗有丝分裂纺锤体装置抗体（MSA）和抗着丝粒抗体（ACA），并探讨其在小细胞肺癌（SCLC）诊断中的临床价值，为SCLC的分子研究提供临床依据。
本研究纳入93例SCLC患者、208例其他癌症患者和50例健康对照。采用酶联免疫吸附测定（ELISA）检测MSA抗体。通过间接免疫荧光法（IIF）检测MSA、ACA和抗核抗体（ANA）。并对结果进行回顾性分析。
①IIF法检测SCLC组MSA和ACA的阳性率分别为36.56%和30.11%，高于其他肿瘤组（P<0.01）；②相关性分析中，MSA与SCLC的RR（相对比值）值高达12.93、12.74，ACA和ANA与SCLC的RR值分别为4.31和3.48；③MSA检测SCLC的ROC（受试者工作特征）曲线下面积（AUC）为0.778，具有中等诊断价值。
MSA和ACA因其高检出率可能成为SCLC的新标志物。这两种标志物可能参与SCLC的发生发展，风险极高。因此，本研究对SCLC的早期检测、临床诊断及潜在治疗具有一定的应用价值。
