乌药（樟科）20对叶绿体微卫星引物的开发
Development of 20 chloroplast microsatellite primers in wuyao (, Lauraceae).
作者信息
Ye Jun-Wei, Li De-Zhu
机构信息
Germplasm Bank of Wild Species Kunming Institute of Botany Chinese Academy of Sciences Kunming 650201 People's Republic of China.
Natural History Research Center of Shanghai Natural History Museum Shanghai Science and Technology Museum Shanghai 200127 People's Republic of China.
出版信息
Appl Plant Sci. 2019 Jul 26;7(8):e01213. doi: 10.1002/aps3.1213. eCollection 2019 Aug.
PREMISE OF THE STUDY
To investigate the population genetics and evolutionary history of wuyao (, Lauraceae) and other species, polymorphic chloroplast microsatellite (cpSSR) primers were developed.
METHODS AND RESULTS
The complete chloroplast genome of was used to design 74 primers for . Agarose gel electrophoresis and Sanger sequencing were used to select 20 polymorphic primers. These primers were then tested in 70 individuals from three populations of . The number of alleles ranged from two to four, and the unbiased haploid diversity index ranged from 0.457 to 0.685. Up to 17 primers successfully amplified in four other species: , , , and var. .
CONCLUSIONS
The 20 primers reported here can potentially be used for future genetic studies of and other species.
研究前提
为了研究乌药（樟科）及其他物种的群体遗传学和进化历史，开发了多态性叶绿体微卫星（cpSSR）引物。
方法与结果
利用乌药的完整叶绿体基因组设计了74对引物。通过琼脂糖凝胶电泳和桑格测序筛选出20对多态性引物。然后在来自乌药三个种群的70个个体中对这些引物进行测试。等位基因数量从2到4不等，无偏单倍体多样性指数从0.457到0.685不等。多达17对引物在其他四种樟科植物中成功扩增：沉水樟、黄樟、毛叶樟和银木变种。
结论
本文报道的20对引物可能可用于未来乌药及其他樟科物种的遗传学研究。
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