PHIM Plant Health Institute, Univ Montpellier, IRD, CIRAD, INRAE, Institut Agro, 34394, Montpellier, France.
Institut Togolais de Recherche Agronomique (ITRA), B.P. 1163, Lomé, Togo.
Arch Virol. 2022 Jan;167(1):245-248. doi: 10.1007/s00705-021-05262-0. Epub 2021 Oct 25.
Pearl millet (Pennisetum glaucum (L.) R. Br.) is a staple food that is widely cultivated in sub-Saharan Africa. In August 2018, a survey was conducted in the main producing regions of Burkina Faso, and leaf samples were analyzed using virion-associated nucleic acid (VANA)-based metagenomic approach and Illumina sequencing. A new virus, tentatively named "Pennisetum glaucum marafivirus" (PGMV), was detected, and its complete nucleotide sequence of 6364 nucleotides was determined. The sequence contains a large open reading frame (ORF) encoding a polyprotein of 224.2 kDa with five domains (methyltransferase, papain-like protease, helicase, RNA-dependent RNA polymerase, and coat proteins), typical of marafiviruses. Additionally, a characteristic conserved marafibox domain was detected in the genome. The nucleotide sequence of the complete PGMV genome shares 68.5% identity with that of sorghum bicolor marafivirus, and its coat protein shares 58.5% identity with that of oat blue dwarf virus. Phylogenetic analysis confirmed that the pearl millet virus is unambiguously grouped with members of the genus Marafivirus in the family Tymoviridae. This is the first report on the occurrence of a marafivirus in pearl millet.
珍珠粟（Pennisetum glaucum (L.) R. Br.）是一种主食，在撒哈拉以南非洲广泛种植。2018 年 8 月，在布基纳法索的主要生产区进行了调查，并使用基于病毒相关核酸（VANA）的宏基因组方法和 Illumina 测序分析了叶片样本。检测到一种新病毒，暂定名为“珍珠粟马拉菲病毒”（PGMV），并确定了其全长 6364 个核苷酸的核苷酸序列。该序列包含一个编码 224.2 kDa 多蛋白的大开放阅读框（ORF），该多蛋白具有五个结构域（甲基转移酶、木瓜蛋白酶样蛋白酶、解旋酶、RNA 依赖性 RNA 聚合酶和外壳蛋白），为马拉菲病毒的典型特征。此外，在基因组中检测到一个特征保守的马拉菲盒结构域。完整 PGMV 基因组的核苷酸序列与高粱马拉菲病毒的核苷酸序列相似度为 68.5%，其外壳蛋白与燕麦蓝矮病毒的相似度为 58.5%。系统进化分析证实，该珍珠粟病毒与呼肠孤病毒科 Tymoviridae 属的成员明确地归为一组。这是首次报道在珍珠粟中发生马拉菲病毒。
