Tao Mingfang, Tasdemir Cemal, Tasdemir Seda, Shahabi Ali, Liu Guiming
Department of Urology, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
Department of Urology, Inonu University, Medical Faculty, Malatya, Turkey.
Int Braz J Urol. 2017 Jul-Aug;43(4):753-761. doi: 10.1590/S1677-5538.IBJU.2016.0454.
Diabetes affects the erectile function significantly. However, the penile alterations in the early stage of diabetes in experimental animal models have not been well studied. We examined the changes of the penis and its main erectile components in diabetic rats.
Male Sprague-Dawley rats were divided into 2 groups: streptozotocin (STZ)-induced diabetics and age-matched controls. Three or nine weeks after diabetes induction, the penis was removed for immunohistochemical staining of smooth muscle and neuronal nitric oxide synthase (nNOS) in midshaft penile tissues. The cross-sectional areas of the whole midshaft penis and the corpora cavernosa were quantified. The smooth muscle in the corpora cavernosa and nNOS in the dorsal nerves were quantified.
The weight, but not the length, of the penis was lower in diabetics. The crosssectional areas of the total midshaft penis and the corpora cavernosa were lower in diabetic rats compared with controls 9 weeks, but not 3 weeks after diabetes induction. The cross-sectional area of smooth muscle in the corpora cavernosa as percentage of the overall area of the corpora cavernosa was lower in diabetic rats than in controls 9 weeks, but not 3 weeks after diabetes induction. Percentage change of nNOS in dorsal nerves was similar at 3 weeks, and has a decreased trend at 9 weeks in diabetic rats compared with controls.
Diabetes causes temporal alterations in the penis, and the significant changes in STZ rat model begin 3-9 weeks after induction. Further studies on the reversibility of the observed changes are warranted.
糖尿病对勃起功能有显著影响。然而，实验动物模型中糖尿病早期阴茎的改变尚未得到充分研究。我们研究了糖尿病大鼠阴茎及其主要勃起组织成分的变化。
将雄性Sprague-Dawley大鼠分为2组：链脲佐菌素（STZ）诱导的糖尿病组和年龄匹配的对照组。糖尿病诱导后3周或9周，取出阴茎进行阴茎中段组织平滑肌和神经元型一氧化氮合酶（nNOS）的免疫组织化学染色。对整个阴茎中段和海绵体的横截面积进行定量分析。对海绵体中的平滑肌和背神经中的nNOS进行定量分析。
糖尿病大鼠阴茎重量降低，但长度未降低。糖尿病大鼠阴茎中段和海绵体的横截面积在糖尿病诱导9周后低于对照组，但3周后无差异。糖尿病大鼠海绵体平滑肌横截面积占海绵体总面积的百分比在糖尿病诱导9周后低于对照组，但3周后无差异。糖尿病大鼠背神经中nNOS的百分比变化在3周时与对照组相似，9周时呈下降趋势。
糖尿病导致阴茎出现暂时性改变，在STZ大鼠模型中，显著变化在诱导后3 - 9周开始出现。对观察到的这些变化的可逆性进行进一步研究是有必要的。
