Department of Urology, University of Health Sciences Bagcilar Training and Research Hospital, Istanbul, Turkey.
Department of Urology, University of Health Sciences Sultan Abdulhamid Han Training and Research Hospital, Istanbul, Turkey.
Int J Impot Res. 2021 Jul;33(5):1-8. doi: 10.1038/s41443-020-0301-1. Epub 2020 May 11.
We aimed to determine the effects of ozone treatment on functional and biochemical changes in corpus cavernosum of diabetic rats. A total of 18 rats were included in the study. The rats were divided into the three groups as control, diabetes mellitus, and diabetes mellitus + ozone therapy groups. In the latter, ozone gas mixture was administered intraperitoneally for 2 weeks after the induction of experimental diabetes model. Erectile response was evaluated by determining mean intracavernosal pressure. Tissue neuronal, inducible and endothelial nitric oxide synthase levels were evaluated with commercial ELISA kits. Immunohistochemical evaluation was also performed to determine the expression levels of nitric oxide synthases semiquantatively. Mean intracavernosal pressure and intracavernosal pressure/systemic arterial blood pressure ratio were significantly higher in the diabetes mellitus + ozone therapy group than those of diabetes mellitus group (24.57 ± 6.36 mmHg vs. 5.98 ± 2.04 mmHg, p = 0.005 and 0.81 ± 0.16 vs. 0.26 ± 0.11, p = 0.0001, respectively). The level of penile tissue endothelial nitric oxide synthase was significantly higher in diabetes mellitus + ozone therapy group compared with others (19.28 ± 3.40 ng/mL vs. 13.47 ± 2.06 ng/mL and 13.28 ± 1.48 ng/mL, P = 0.01). Endothelial nitric oxide synthase expression increased significantly with ozone therapy. Our results suggest that ozone therapy may be beneficial in reducing the negative effects of diabetes on erectile dysfunction as a result of enhanced enzymatic activity in endothelial nitric oxide synthase levels.
我们旨在确定臭氧处理对糖尿病大鼠海绵体功能和生化变化的影响。共有 18 只大鼠纳入研究。将大鼠分为三组：对照组、糖尿病组和糖尿病+臭氧治疗组。在后一组中，在实验性糖尿病模型诱导后，通过腹腔内给予臭氧混合气体 2 周。通过测定平均海绵体内压来评估勃起反应。使用商业 ELISA 试剂盒评估组织神经元、诱导型和内皮型一氧化氮合酶水平。还进行了免疫组织化学评估，以半定量确定一氧化氮合酶的表达水平。糖尿病+臭氧治疗组的平均海绵体内压和海绵体内压/系统动脉血压比值明显高于糖尿病组（24.57±6.36mmHg 比 5.98±2.04mmHg，p=0.005 和 0.81±0.16 比 0.26±0.11，p=0.0001）。与其他两组相比，糖尿病+臭氧治疗组的阴茎组织内皮型一氧化氮合酶水平明显升高（19.28±3.40ng/mL 比 13.47±2.06ng/mL 和 13.28±1.48ng/mL，P=0.01）。内皮型一氧化氮合酶的表达随着臭氧治疗而显著增加。我们的结果表明，臭氧治疗可能有益于减少糖尿病对勃起功能障碍的负面影响，这是由于内皮型一氧化氮合酶水平的酶活性增强所致。
