Arpag Huseyin, Gül Mehmet, Aydemir Yusuf, Atilla Nurhan, Yiğitcan Birgül, Cakir Tugrul, Polat Cemal, Þehirli Özer, Sayan Muhammet
a Department of Chest Disease , Kahramanmaras Sutcu Imam University Medical Faculty , Kahramanmaras , Turkey.
b Department of Histology , Malatya Inonu University Medical Faculty , Malatya , Turkey.
J Invest Surg. 2018 Apr;31(2):107-113. doi: 10.1080/08941939.2017.1296513. Epub 2017 Mar 24.
Oxidative stress is one of the major causes of methotrexate induced lung injury (MILI). Alpha-lipoic acid (ALA), which occurs naturally in human food, has antioxidative and anti-inflammatory activities. The aim of this study was to research the potential protective role of ALA on MILI in rats.
Twenty one rats were randomly subdivided into three groups: control (group I), methotrexate (MTX) treated (group II), and MTX+ALA treated (group III). Lung injury was performed with a single dose of MTX (20 mg/kg) to groups 2 and 3. On the sixth day, animals in all groups were sacrificed by decapitation and lung tissue and blood samples were removed for histological examination and also measurement the levels of interleukin-1-beta (IL-1β), malondialdehyde (MDA), glutathione (GSH), tumour necrosis factor-alpha (TNF-α), myeloperoxidase (MPO), and sodium potassium-adenosine triphosphatase (Na+/K+ATPase).
In MTX group tissue GSH, Na+/K+ATPase activities were lower, tissue MDA, MPO and plasma IL-1?, TNF-? were significantly higher than the other groups. Histopathological examination showed that lung injury was less severe in group 2 according to group 3.
Oxidative damage of MTX in rat lung is partially reduced when combined with ALA.
氧化应激是甲氨蝶呤诱导的肺损伤（MILI）的主要原因之一。α-硫辛酸（ALA）天然存在于人类食物中，具有抗氧化和抗炎活性。本研究旨在探讨ALA对大鼠MILI的潜在保护作用。
将21只大鼠随机分为三组：对照组（I组）、甲氨蝶呤（MTX）治疗组（II组）和MTX+ALA治疗组（III组）。对第2组和第3组大鼠单次给予MTX（20mg/kg）造成肺损伤。在第6天，通过断头处死所有组的动物，取出肺组织和血液样本进行组织学检查，并测量白细胞介素-1β（IL-1β）、丙二醛（MDA）、谷胱甘肽（GSH）、肿瘤坏死因子-α（TNF-α）、髓过氧化物酶（MPO）和钠钾-三磷酸腺苷酶（Na+/K+ATPase）的水平。
MTX组组织GSH、Na+/K+ATPase活性较低，组织MDA、MPO以及血浆IL-1β、TNF-α显著高于其他组。组织病理学检查显示，与第3组相比，第2组肺损伤较轻。
MTX与ALA联合使用时，大鼠肺的氧化损伤部分减轻。
