Ankara University, Faculty of Science, Department of Chemistry, Ankara, Turkey.
Ankara University, Faculty of Science, Department of Chemistry, Ankara, Turkey.
Talanta. 2017 May 15;167:286-295. doi: 10.1016/j.talanta.2017.02.021. Epub 2017 Feb 10.
A comparison of the analytical performances of two xanthine biosensors, based on the use of different metal oxide nanoparticles (MONPs: CoO or FeO)-modified carboxylated multiwalled carbon nanotubes (c-MWCNTs)-7,7',8,8'-tetracyanoquinodimethane (TCNQ)-chitosan (CHIT) composite, is discussed. Xanthine oxidase (XOD) enzyme was covalently attached to c-MWCNTs/MONPs/TCNQ/CHIT/GCE via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) chemistry and the electrode surface was further modified with Nafion in order to minimize the effect of possible interfering substances. The results showed that analytical performance of the FeO based biosensor was better than the CoO based biosensor. The linear working range, limit of detection and sensitivity were found to be 1.9×10-2.3×10M, 0.20μM (S/N=3), 25.07μAmMcm for the FeO based biosensor and 1.9×10-1.2×10M, 0.36μM (S/N=3), 13.24μAmMcm for the CoO based biosensor, respectively. The purposed biosensors were applied in the determination of xanthine in coffee samples, and satisfactory results were obtained.
两种基于不同金属氧化物纳米粒子（MONPs：CoO 或 FeO）修饰的羧基多壁碳纳米管（c-MWCNTs）-7,7',8,8'-四氰基对醌二甲烷（TCNQ）-壳聚糖（CHIT）复合材料的黄嘌呤生物传感器的分析性能进行了比较。黄嘌呤氧化酶（XOD）酶通过 N-乙基-N'-(3-二甲基氨基丙基)碳二亚胺（EDC）和 N-羟基琥珀酰亚胺（NHS）化学共价连接到 c-MWCNTs/MONPs/TCNQ/CHIT/GCE 上，并进一步用 Nafion 修饰电极表面，以最大程度地减少可能的干扰物质的影响。结果表明，FeO 基生物传感器的分析性能优于 CoO 基生物传感器。FeO 基生物传感器的线性工作范围、检测限和灵敏度分别为 1.9×10-2.3×10M、0.20μM（S/N=3）和 25.07μAmMcm，而 CoO 基生物传感器的线性工作范围、检测限和灵敏度分别为 1.9×10-1.2×10M、0.36μM（S/N=3）和 13.24μAmMcm。所提出的生物传感器用于测定咖啡样品中的黄嘌呤，获得了令人满意的结果。
