基于多壁碳纳米管和金纳米粒子的新型灵敏安培胆碱生物传感器。
A novel sensitive amperometric choline biosensor based on multiwalled carbon nanotubes and gold nanoparticles.
机构信息
Applied Organic Chemistry Department, National Research Centre, Giza, Egypt; Department of Chemistry, Faculty of Sciences and Technology, University of Coimbra, 3004-535 Coimbra, Portugal.
Department of Chemistry, Faculty of Sciences and Technology, University of Coimbra, 3004-535 Coimbra, Portugal.
出版信息
Talanta. 2017 May 15;167:462-469. doi: 10.1016/j.talanta.2017.02.048. Epub 2017 Feb 21.
A novel amperometric biosensor for choline determination has been developed, exploiting the electrocatalytic properties of multiwalled carbon nanotubes (MWCNT) and gold nanoparticles (GNP). Chitosan (Chit), a natural biocompatible polymer, was used to disperse CNT, then Chit-MWCNT was dropped on the surface of a glassy carbon electrode (GCE), followed by GNP; finally, choline oxidase (ChOx) was immobilized by glutaraldehyde crosslinking. The ChOx/(GNP)/MWCNT/GCE exhibited linear response to choline from 3 to 120µM, the sensitivity was 204µAcmmM and the detection limit was 0.6μM. The biosensor exhibited good intra and inter-electrode precision, and excellent selectivity and stability. Electrochemical impedance spectroscopy (EIS) was also used to measure choline at 0.0V and this is the first report on choline determination by EIS. Successful measurement in milk samples was performed.
一种用于测定胆碱的新型电流式生物传感器已经被开发出来，该传感器利用了多壁碳纳米管（MWCNT）和金纳米粒子（GNP）的电催化性能。壳聚糖（Chit）是一种天然的生物相容性聚合物，被用来分散 CNT，然后将 Chit-MWCNT 滴在玻碳电极（GCE）的表面，接着是 GNP；最后，通过戊二醛交联固定胆碱氧化酶（ChOx）。ChOx/（GNP）/MWCNT/GCE 对 3 至 120µM 的胆碱呈现线性响应，灵敏度为 204µAcmmM，检测限为 0.6μM。该生物传感器表现出良好的内电极和外电极精度，以及优异的选择性和稳定性。电化学阻抗谱（EIS）也被用于在 0.0V 下测量胆碱，这是首次通过 EIS 报告的胆碱测定。在牛奶样品中成功进行了测量。
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