人成纤维细胞干扰素mRNA的诱导与衰减
Induction and decay of human fibroblast interferon mRNA.
作者信息
Cavalieri R L, Havell E A, Vilcek J, Pestka S
出版信息
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4415-9. doi: 10.1073/pnas.74.10.4415.
Polyadenylylated interferon mRNA, obtained from induced human fibroblasts, was quantitatively assayed by synthesis of biologically active human interferon in Xenopus laevis oocytes. The assay for interferon mRNA was used to distinguish between various hypotheses relating to interferon induction and biosynthesis. The data demonstrate that on induction with poly(I-poly(C) human fibroblasts accumulate interferon mRNA for 1-1.5 hr, after which time the mRNA is rapidly degraded with a half-life (t 1/2) of 18 min. Treatment of cells with cycloheximide prolongs the period of accumulation to 3 hr and decreases the rate of mRNA inactivation (t 1/2 = 49 min). Treatment with actinomycin D decreases the rate of inactivation still further (t 1/2 = 68 min). A comparison of cellular interferon synthesis with the relative amounts of interferon m RNA after simple induction or inductionin the presence of the inhibitors (superinduction) indicated a general correlation. Thus, on induction, the genes for interferon are activated to produce a transcript for a short time. The superinducing treatments prolong the period of accumulation and decrease the rate of degradation of this transcript.
从诱导的人成纤维细胞中获得的聚腺苷酸化干扰素mRNA，通过在非洲爪蟾卵母细胞中合成具有生物活性的人干扰素进行定量测定。干扰素mRNA的测定用于区分与干扰素诱导和生物合成相关的各种假说。数据表明，用聚肌苷酸-聚胞苷酸（poly(I-poly(C))）诱导后，人成纤维细胞积累干扰素mRNA达1 - 1.5小时，此后mRNA迅速降解，半衰期（t1/2）为18分钟。用环己酰亚胺处理细胞可将积累期延长至3小时，并降低mRNA失活速率（t1/2 = 49分钟）。用放线菌素D处理可进一步降低失活速率（t1/2 = 68分钟）。比较简单诱导或在抑制剂存在下诱导（超诱导）后细胞干扰素合成与干扰素mRNA的相对量，显示出总体相关性。因此，诱导时，干扰素基因被激活以在短时间内产生转录本。超诱导处理延长了积累期并降低了该转录本的降解速率。
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