Cavalieri R L, Havell E A, Vilcek J, Pestka S
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3287-91. doi: 10.1073/pnas.74.8.3287.
Human fibroblasts and leukocytes produce interferons which may be distinguished by their antigenic and species specificity as well as by their molecular weight distributions. To elucidate the basis for these differences, we isolated mRNA from induced human fibroblasts and lymphoblastoid (Namalva) cells and studied the products of translation in Xenopus laevis oocytes. The mRNA from the respective cells yielded translation products, in oocytes, that were characteristic of the cells from which the mRNA was derived. We conclude that human cells contain at least two structural genes for interferon, coding for polypeptides differing in primary sequence. Fibroblasts synthesize a single species of interferon; lymphoblastoid cells synthesize two species, the fibroblast and leukocyte types.
人类成纤维细胞和白细胞可产生干扰素，这些干扰素可通过其抗原特异性、物种特异性以及分子量分布来区分。为了阐明这些差异的基础，我们从诱导的人类成纤维细胞和淋巴母细胞样（Namalva）细胞中分离出信使核糖核酸（mRNA），并研究了非洲爪蟾卵母细胞中的翻译产物。来自相应细胞的mRNA在卵母细胞中产生的翻译产物具有该mRNA来源细胞的特征。我们得出结论，人类细胞至少含有两种干扰素结构基因，编码一级序列不同的多肽。成纤维细胞合成单一类型的干扰素；淋巴母细胞样细胞合成两种类型的干扰素，即成纤维细胞型和白细胞型。
