非洲爪蟾卵母细胞合成人干扰素：人类细胞中干扰素的两个结构基因。
Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells.
作者信息
Cavalieri R L, Havell E A, Vilcek J, Pestka S
出版信息
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3287-91. doi: 10.1073/pnas.74.8.3287.
Abstract
Human fibroblasts and leukocytes produce interferons which may be distinguished by their antigenic and species specificity as well as by their molecular weight distributions. To elucidate the basis for these differences, we isolated mRNA from induced human fibroblasts and lymphoblastoid (Namalva) cells and studied the products of translation in Xenopus laevis oocytes. The mRNA from the respective cells yielded translation products, in oocytes, that were characteristic of the cells from which the mRNA was derived. We conclude that human cells contain at least two structural genes for interferon, coding for polypeptides differing in primary sequence. Fibroblasts synthesize a single species of interferon; lymphoblastoid cells synthesize two species, the fibroblast and leukocyte types.
摘要
人类成纤维细胞和白细胞可产生干扰素，这些干扰素可通过其抗原特异性、物种特异性以及分子量分布来区分。为了阐明这些差异的基础，我们从诱导的人类成纤维细胞和淋巴母细胞样（Namalva）细胞中分离出信使核糖核酸（mRNA），并研究了非洲爪蟾卵母细胞中的翻译产物。来自相应细胞的mRNA在卵母细胞中产生的翻译产物具有该mRNA来源细胞的特征。我们得出结论，人类细胞至少含有两种干扰素结构基因，编码一级序列不同的多肽。成纤维细胞合成单一类型的干扰素；淋巴母细胞样细胞合成两种类型的干扰素，即成纤维细胞型和白细胞型。
相似文献
1
Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells.非洲爪蟾卵母细胞合成人干扰素：人类细胞中干扰素的两个结构基因。
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3287-91. doi: 10.1073/pnas.74.8.3287.
2
Synthesis of interferon in heterologous cells, cell-free extracts, and Xenopus laevis oocytes.干扰素在异源细胞、无细胞提取物及非洲爪蟾卵母细胞中的合成。
Tex Rep Biol Med. 1977;35:117-25.
3
Interferon activity produced by translation of human interferon messenger RNA in cell-free ribosomal systems and in Xenopus oöcytes.在无细胞核糖体系统和非洲爪蟾卵母细胞中通过人干扰素信使核糖核酸的翻译所产生的干扰素活性。
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4881-5. doi: 10.1073/pnas.72.12.4881.
4
Heterogeneity of interferon mRNA species from Sendai virus-induced human lymphoblastoid (Namalva) cells and Newcastle disease virus-induced murine fibroblastoid (L) cells.仙台病毒诱导的人淋巴母细胞样（Namalva）细胞和新城疫病毒诱导的鼠成纤维细胞样（L）细胞中干扰素mRNA种类的异质性。
Nucleic Acids Res. 1981 Jan 10;9(1):149-60. doi: 10.1093/nar/9.1.149.
5
Does 3'-terminal poly(A) stabilize human fibroblast interferon mRNA in oocytes of Xenopus laevis?3'末端的聚腺苷酸（poly(A)）是否能稳定非洲爪蟾卵母细胞中的人成纤维细胞干扰素mRNA？
Proc Natl Acad Sci U S A. 1978 Oct;75(10):5030-3. doi: 10.1073/pnas.75.10.5030.
6
Translation of human interferon mRNAs in Xenopus laevis oocytes.人干扰素mRNA在非洲爪蟾卵母细胞中的翻译。
Methods Enzymol. 1981;79(Pt B):68-71. doi: 10.1016/s0076-6879(81)79014-1.
7
Characterization of interferon messenger RNA synthesis in Namalva cells.纳马鲁瓦细胞中干扰素信使核糖核酸合成的特性研究
J Biol Chem. 1980 Apr 10;255(7):2955-61.
8
Le interferon mRNA from human fibroblasts.来自人成纤维细胞的γ干扰素信使核糖核酸。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5341-5. doi: 10.1073/pnas.77.9.5341.
9
Translation of human immune interferon messenger RNA in Xenopus laevis oocytes.
Biochem Biophys Res Commun. 1981 May 29;100(2):865-71. doi: 10.1016/s0006-291x(81)80253-7.
10
Isolation and cell-free translation of human interferon mRNA from fibroblasts and leukocytes.从成纤维细胞和白细胞中分离人干扰素信使核糖核酸并进行无细胞翻译
Methods Enzymol. 1981;79(Pt B):51-9. doi: 10.1016/s0076-6879(81)79012-8.
引用本文的文献
1
Aimez-vous Brahms? A story capriccioso from the discovery of a cytokine family and its regulators.你喜欢勃拉姆斯吗？一个关于细胞因子家族及其调节因子发现的随想曲故事。
Nat Immunol. 2009 May;10(5):447-9. doi: 10.1038/ni0509-447.
2
PEGylated interferon-alpha2b: a branched 40K polyethylene glycol derivative.聚乙二醇化干扰素-α2b：一种分支状40K聚乙二醇衍生物。
Pharm Res. 2005 Aug;22(8):1374-86. doi: 10.1007/s11095-005-5278-4. Epub 2005 Aug 3.
3
Constitutive production of alpha and beta interferons in mutant human cell lines.突变型人细胞系中α和β干扰素的组成型产生。
J Virol. 1994 Jun;68(6):4057-62. doi: 10.1128/JVI.68.6.4057-4062.1994.
4
Human natural interferon-alpha producing cells.人类天然产生α干扰素的细胞。
Pharmacol Ther. 1993 Oct;60(1):39-62. doi: 10.1016/0163-7258(93)90021-5.
5
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.功能性信使核糖核酸通过克隆的互补脱氧核糖核酸的SP6体外转录产生。
Nucleic Acids Res. 1984 Sep 25;12(18):7057-70. doi: 10.1093/nar/12.18.7057.
6
Cell cultures producing human interferon.生产人干扰素的细胞培养物。
Antimicrob Agents Chemother. 1981 Jul;20(1):1-4. doi: 10.1128/AAC.20.1.1.
7
Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes.从人淋巴细胞中提取的γ（免疫）干扰素信使核糖核酸的部分特性分析
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3469-72. doi: 10.1073/pnas.78.6.3469.
8
Translational activity and functional stability of human fibroblast beta 1 and beta 2 interferon mRNAs lacking 3'-terminal RNA sequences.缺乏3'-末端RNA序列的人成纤维细胞β1和β2干扰素mRNA的翻译活性和功能稳定性。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1741-5. doi: 10.1073/pnas.78.3.1741.
9
Construction and identification of bacterial plasmids containing nucleotide sequence for human leukocyte interferon.含人白细胞干扰素核苷酸序列细菌质粒的构建与鉴定
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7010-3. doi: 10.1073/pnas.77.12.7010.
10
The absence of introns within a human fibroblast interferon gene.人类成纤维细胞干扰素基因内不存在内含子。
Nucleic Acids Res. 1981 Jan 24;9(2):247-66. doi: 10.1093/nar/9.2.247.
本文引用的文献
1
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
2
Translational capacity of living frog eggs and oocytes, as judged by messenger RNA injection.通过信使核糖核酸注射判断的活蛙卵和卵母细胞的翻译能力。
J Mol Biol. 1971 Oct 14;61(1):93-103. doi: 10.1016/0022-2836(71)90208-7.
3
Subunit structure and number of combining sites of the immunoglobulin A myeloma protein produced by mouse plasmacytoma MOPC-315.小鼠浆细胞瘤MOPC - 315产生的免疫球蛋白A骨髓瘤蛋白的亚基结构和结合位点数量
Biochemistry. 1971 Nov 23;10(24):4359-68. doi: 10.1021/bi00800a002.
4
Interferon induction and action.干扰素的诱导与作用。
Annu Rev Microbiol. 1971;25:333-60. doi: 10.1146/annurev.mi.25.100171.002001.
5
6
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.利用蛙卵和卵母细胞研究信使核糖核酸及其在活细胞中的翻译。
Nature. 1971 Sep 17;233(5316):177-82. doi: 10.1038/233177a0.
7
Amino acid sequence of the light chain of a mouse myeloma protein (MOPC-315).一种小鼠骨髓瘤蛋白（MOPC - 315）轻链的氨基酸序列。
Biochemistry. 1973 Dec 18;12(26):5400-16.
8
Interferon messenger RNA: translation in heterologous cells.干扰素信使核糖核酸：在异源细胞中的翻译
Proc Natl Acad Sci U S A. 1972 May;69(5):1203-7. doi: 10.1073/pnas.69.5.1203.
9
Purification of 14S messenger RNA of immunoglobulin light chain that codes for a possible light-chain precursor.编码一种可能的轻链前体的免疫球蛋白轻链14S信使核糖核酸的纯化。
Proc Natl Acad Sci U S A. 1973 Feb;70(2):451-5. doi: 10.1073/pnas.70.2.451.
10
Calf crystallin synthesis in frog cells: the translation of lens-cell 14S RNA in oocytes.蛙细胞中牛晶体蛋白的合成：卵母细胞中晶状体细胞14S RNA的翻译。
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1606-9. doi: 10.1073/pnas.69.6.1606.
Zotero 插件