• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人乳可刺激培养的成纤维细胞中的DNA合成和细胞增殖。

Human milk stimulates DNA synthesis and cellular proliferation in cultured fibroblasts.

作者信息

Klagsbrun M

出版信息

Proc Natl Acad Sci U S A. 1978 Oct;75(10):5057-61. doi: 10.1073/pnas.75.10.5057.

DOI:10.1073/pnas.75.10.5057
PMID:283415
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336262/
Abstract

Human milk contains a mitogenic factor that stimulates DNA synthesis and cell division in mouse and human fibroblasts in vitro. Milk at a concentration of 1% (vol/vol) is as active in stimulating DNA synthesis as is 5% (vol/vol) human serum and 10% (vol/vol) calf serum. The mitogenic activity of human milk is destroyed by incubation with trypsin and chymotrypsin. However, neither urea, guanidine hydrochloride-dithiothreitol, nor exposure to pH 1 will inactivate the milk-derived growth factor. Gel filtration and isoelectric focusing indicate that the mitogenic activity of human milk has a molecular weight between 14,000 and 18,000 and an isoelectric point between 4.4 and 4.7.

摘要

人乳中含有一种促有丝分裂因子，可在体外刺激小鼠和人成纤维细胞中的DNA合成及细胞分裂。浓度为1%（体积/体积）的人乳在刺激DNA合成方面与5%（体积/体积）的人血清和10%（体积/体积）的小牛血清一样活跃。人乳的促有丝分裂活性在与胰蛋白酶和糜蛋白酶一起孵育后会被破坏。然而，尿素、盐酸胍 - 二硫苏糖醇，以及暴露于pH 1环境均不会使源自人乳的生长因子失活。凝胶过滤和等电聚焦表明，人乳的促有丝分裂活性物质分子量在14,000至18,000之间，等电点在4.4至4.7之间。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29d2/336262/fc680a2eb25d/pnas00669-0432-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29d2/336262/fc680a2eb25d/pnas00669-0432-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29d2/336262/fc680a2eb25d/pnas00669-0432-a.jpg

相似文献

1
Human milk stimulates DNA synthesis and cellular proliferation in cultured fibroblasts.人乳可刺激培养的成纤维细胞中的DNA合成和细胞增殖。
Proc Natl Acad Sci U S A. 1978 Oct;75(10):5057-61. doi: 10.1073/pnas.75.10.5057.
2
Isolation of colony stimulating factor from human milk.从人乳中分离集落刺激因子。
Biochem Biophys Res Commun. 1983 Jul 29;114(2):797-803. doi: 10.1016/0006-291x(83)90852-5.
3
The effect of human milk on DNA synthesis of neonatal rat hepatocytes in primary culture.人乳对原代培养新生大鼠肝细胞DNA合成的影响。
Pediatr Res. 1991 Mar;29(3):251-5. doi: 10.1203/00006450-199103000-00006.
4
In vitro growth-stimulatory property of pigeon milk.鸽乳的体外生长刺激特性。
Biochem Cell Biol. 1993 May-Jun;71(5-6):303-7. doi: 10.1139/o93-045.
5
Human prostatic cancer cells, PC3, elaborate mitogenic activity which selectively stimulates human bone cells.人前列腺癌细胞PC3具有促有丝分裂活性，能选择性地刺激人骨细胞。
Cancer Res. 1990 Nov 1;50(21):6902-7.
6
Synergistic stimulation of Swiss mouse 3T3 fibroblasts by epidermal growth factor and other factors in human mammary secretions.表皮生长因子与人类乳腺分泌物中的其他因子对瑞士小鼠3T3成纤维细胞的协同刺激作用。
J Endocrinol. 1987 Jan;112(1):151-9. doi: 10.1677/joe.0.1120151.
7
Isolation of a cationic polypeptide from human serum that stimulates proliferation of 3T3 cells.从人血清中分离出一种可刺激3T3细胞增殖的阳离子多肽。
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2635-9. doi: 10.1073/pnas.72.7.2635.
8
A platelet factor stimulating the proliferation of vascular endothelial cells. Partial purification and characterization.一种刺激血管内皮细胞增殖的血小板因子。部分纯化及特性鉴定。
Exp Cell Res. 1985 Aug;159(2):487-94. doi: 10.1016/s0014-4827(85)80022-7.
9
Circulating factors controlling cell proliferation.
Prog Clin Biol Res. 1976;5:193-215.
10
Growth stimulating activity from lysed cultured arterial smooth muscle cells and skin fibroblasts.来自裂解的培养动脉平滑肌细胞和皮肤成纤维细胞的生长刺激活性。
Med Biol. 1985;63(4):175-81.

引用本文的文献

1
Breast milk hormones and their protective effect on obesity.母乳中的激素及其对肥胖的保护作用。
Int J Pediatr Endocrinol. 2009;2009:327505. doi: 10.1155/2009/327505. Epub 2009 Nov 4.
2
Minimal enteral nutrition.少量肠内营养
Indian J Pediatr. 2008 Mar;75(3):267-9. doi: 10.1007/s12098-008-0057-y.
3
Minimal enteral nutrition.微量肠内营养

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Crystalline trypsin inhibitor from colostrum.初乳中的结晶胰蛋白酶抑制剂。
J Biol Chem. 1951 Jun;190(2):563-73.
3
Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal.从小鼠颌下腺中分离出一种可加速新生动物门齿萌出和睁眼的蛋白质。
Indian J Pediatr. 2001 Dec;68(12):1159-60. doi: 10.1007/BF02722935.
4
Insulin-like growth factors in milk and mammary gland.牛奶和乳腺中的胰岛素样生长因子。
J Mammary Gland Biol Neoplasia. 1996 Jul;1(3):297-306. doi: 10.1007/BF02018082.
5
Milk-derived growth factors as serum supplements for the growth of fibroblast and epithelial cells.源自牛奶的生长因子作为成纤维细胞和上皮细胞生长的血清补充剂。
In Vitro Cell Dev Biol Anim. 1995 Nov;31(10):752-60. doi: 10.1007/BF02634116.
6
Studies on the growth-stimulatory activity of pigeon milk--comparison and synergistic effects with serum.鸽乳生长刺激活性的研究——与血清的比较及协同效应
J Comp Physiol B. 1993;163(4):332-6. doi: 10.1007/BF00347784.
7
Bovine colostrum supports the serum-free proliferation of epithelial cells but not of fibroblasts in long-term culture.牛初乳在长期培养中支持上皮细胞而非成纤维细胞的无血清增殖。
J Cell Biol. 1980 Mar;84(3):808-14. doi: 10.1083/jcb.84.3.808.
8
Sertoli cells contain a mitogenic polypeptide.支持细胞含有一种促有丝分裂多肽。
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4774-8. doi: 10.1073/pnas.77.8.4774.
9
Endothelial cell mitogens derived from retina and hypothalamus: biochemical and biological similarities.源自视网膜和下丘脑的内皮细胞促细胞分裂剂：生化及生物学特性的相似性
J Cell Biol. 1984 Oct;99(4 Pt 1):1545-9. doi: 10.1083/jcb.99.4.1545.
10
Inhibition of the binding of 125I-labelled epidermal growth factor to mouse cells by a mitogen in goat mammary secretions.山羊乳腺分泌物中的一种促细胞分裂剂对125I标记的表皮生长因子与小鼠细胞结合的抑制作用。
Biochem J. 1983 May 15;212(2):465-72. doi: 10.1042/bj2120465.
J Biol Chem. 1962 May;237:1555-62.
4
The initiation of cell division in a contact-inhibited mammalian cell line.接触抑制的哺乳动物细胞系中细胞分裂的起始
J Cell Physiol. 1965 Dec;66(3):325-33. doi: 10.1002/jcp.1030660310.
5
Proteolytic enzymes initiating cell division and escape from contact inhibition of growth.启动细胞分裂并逃避生长接触抑制的蛋白水解酶。
Nature. 1970 Jul 11;227(5254):170-1. doi: 10.1038/227170a0.
6
A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts.一种从大鼠肝细胞条件培养液中获得的部分纯化的多肽组分，对胚胎成纤维细胞具有增殖刺激活性。
J Cell Physiol. 1973 Apr;81(2):153-60. doi: 10.1002/jcp.1040810204.
7
Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth.一种成纤维细胞生长因子的定位及其单独作用以及与氢化可的松共同作用对3T3细胞生长的影响。
Nature. 1974 May 10;249(453):123-7. doi: 10.1038/249123a0.
8
The partial purification from calf serum of a fraction with multiplication-stimulating activity for chicken fibroblasts in cell culture and with non-suppressible insulin-like activity.从小牛血清中部分纯化出一种在细胞培养中对鸡成纤维细胞具有增殖刺激活性且具有不可抑制的胰岛素样活性的组分。
J Cell Physiol. 1972 Jun;79(3):319-30. doi: 10.1002/jcp.1040790302.
9
A platelet factor stimulating human normal glial cells.一种刺激人正常神经胶质细胞的血小板因子。
Exp Cell Res. 1976 Mar 1;98(1):170-4. doi: 10.1016/0014-4827(76)90476-6.
10
Purification of a fibroblast growth factor from bovine pituitary.从牛垂体中纯化一种成纤维细胞生长因子。
J Biol Chem. 1975 Apr 10;250(7):2515-20.