华氏巨球蛋白血症患者血清中κ链单克隆免疫球蛋白M的交叉反应独特型

Crossreacting idiotypes of kappa-monoclonal immunoglobulins M in sera of patients with Waldenström's macroglobulinemia.

作者信息

Wehmeier U, Rilke H, Patzold U, Schedel I, Deicher H

机构信息

Abteilung Immunologie und Transfusionsmedizin, Medizinischen Hochschule Hannover, Federal Republic of Germany.

出版信息

Immunobiology. 1987 Dec;176(1-2):144-53. doi: 10.1016/s0171-2985(87)80106-7.

DOI:10.1016/s0171-2985(87)80106-7

PMID:2834288

Abstract

Seventeen anti-idiotypic antisera (anti-Id) were prepared against kappa-monoclonal IgM from patients with Waldenström's macroglobulinemia. Their reactivity against homologous and heterologous IgM was tested using an ELISA. Crossreacting idiotypes were only found in two out of 289 investigated antigen-antibody reactions. One anti-Id IgG crossreacted with determinants on polyclonal IgM. These rare crossreactions were observed in Waldenström's macroglobulinemia patients with and without polyneuropathy. The scarcity of common idiotypes on different monoclonal IgM does, however, not constitute a decisive argument against common or related antibody specificities of such monoclonal IgM.

摘要

针对瓦尔登斯特伦巨球蛋白血症患者的κ-单克隆IgM制备了17种抗独特型抗血清（抗Id）。使用酶联免疫吸附测定法（ELISA）检测它们对同源和异源IgM的反应性。在289次研究的抗原-抗体反应中，仅在其中两例中发现了交叉反应性独特型。一种抗Id IgG与多克隆IgM上的决定簇发生交叉反应。在有和没有多发性神经病的瓦尔登斯特伦巨球蛋白血症患者中均观察到了这些罕见的交叉反应。然而，不同单克隆IgM上常见独特型的稀缺性并不构成反对此类单克隆IgM具有共同或相关抗体特异性的决定性论据。