Han Runhua, Perepelov Andrei V, Wang Yuanyuan, Filatov Andrei V, Wang Min, Shashkov Alexander S, Wang Lei, Knirel Yuriy A
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China.
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
Carbohydr Res. 2017 Apr 18;443-444:49-52. doi: 10.1016/j.carres.2017.02.006. Epub 2017 Feb 28.
On mild acid degradation of the lipopolysaccharide of Enterobacter cloacae C5529, the O-polysaccharide chain was cleaved at the linkages of 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (di-N-acetylpseudaminic acid, Psep5Ac7Ac). The resultant oligosaccharide and an alkali-treated lipopolysaccharide were studied by sugar analysis along with H and C NMR spectroscopy, and the following structure of the tetrasaccharide repeating unit of the O-polysaccharide was established: →4)-β-Psep5Ac7Ac-(2 → 3)-β-d-Galp-(1 → 6)-β-d-Galf-(1 → 3)-α-d-Galp-(1→ It differs from a structurally related O-polysaccharide of E. cloacae G3045 studied early (Perepelov, A. V.; Wang, M.; Filatov, A. V.; Guo, X.; Shashkov, A. S.; Wang, L.; Knirel, Y. A. Carbohydr. Res. 2015; 407:59-62) in positions of substitution of β-Psep5Ac7Ac (O-4 vs. O-8) and β-Galp (O-3 vs. O-6) and the absence of a side-chain α-Galp residue. The O-antigen gene clusters of E. cloacae C5529 and G3045 are organized identically and include genes with the same putative functions in the O-polysaccharide synthesis. Based on these and serological data, it is suggested to combine E. cloacae C5529 and G3054 in one O-serogroup as two subgroups.
在阴沟肠杆菌C5529脂多糖的轻度酸降解过程中，O-多糖链在5,7-二乙酰氨基-3,5,7,9-四脱氧-L-甘油-L-甘露糖-壬-2-酮糖酸（二-N-乙酰假氨基糖酸，Psep5Ac7Ac）的连接位点处断裂。通过糖分析以及氢和碳核磁共振光谱对所得寡糖和碱处理的脂多糖进行了研究，并确定了O-多糖四糖重复单元的以下结构：→4)-β-Psep5Ac7Ac-(2→3)-β-d-半乳糖-(1→6)-β-d-半乳糖醛酸-(1→3)-α-d-半乳糖-(1→ 它与早期研究的阴沟肠杆菌G3045的一种结构相关的O-多糖（佩列佩洛夫，A. V.；王，M.；菲拉托夫，A. V.；郭，X.；沙什科夫，A. S.；王，L.；克尼雷尔，Y. A.《碳水化合物研究》2015年；407:59 - 62）在β-Psep5Ac7Ac的取代位置（O-4与O-8）和β-半乳糖（O-3与O-6）以及缺少侧链α-半乳糖残基方面存在差异。阴沟肠杆菌C5529和G3045的O-抗原基因簇组织方式相同，并且在O-多糖合成中包含具有相同假定功能的基因。基于这些以及血清学数据，建议将阴沟肠杆菌C5529和G3054合并为一个O-血清群的两个亚群。
