N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, PR China; Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, TEDA, Tianjin 300457, PR China.
Carbohydr Res. 2014 Mar 31;387:10-3. doi: 10.1016/j.carres.2014.01.001. Epub 2014 Jan 14.
The O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G2277 and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the linear pentasaccharide repeating unit was established, where a galacturonic acid (GalA) residue is mono-O-acetylated at position either 2 or 3: The O-antigen gene cluster of E. cloacae G2277 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure.
肠杆菌 G2277 的 O-多糖通过脂多糖的温和酸降解分离，并通过糖分析以及 1D 和 2D（1）H 和（13）C NMR 光谱进行研究。建立了线性五糖重复单元的以下结构，其中半乳糖醛酸（GalA）残基在位置 2 或 3 处单-O-乙酰化：肠杆菌 G2277 的 O-抗原基因簇进行了测序。通过与现有数据库中的序列进行比较，推测基因功能，并与 O-多糖结构一致。
