The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China; TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
Carbohydr Res. 2022 Sep;519:108612. doi: 10.1016/j.carres.2022.108612. Epub 2022 Jun 9.
The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G2559 and studied by sugar analysis along with 1D and 2D H and C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established. The O-antigen gene cluster of Enterobacter cloacae G2559 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-antigen structure.
肠杆菌 G2559 的 O-多糖（OPS）通过脂多糖的弱酸降解分离得到，并通过糖分析以及 1D 和 2D H 和 C NMR 光谱进行了研究。确定了支化五糖重复单元的以下结构。测序了肠杆菌 G2559 的 O-抗原基因簇。通过与现有数据库中的序列比较，推测了基因的功能，并与 O-抗原结构完全一致。
