阴沟肠杆菌C4115 O抗原的结构与基因簇
Structure and gene cluster of the O-antigen of Enterobacter cloacae C4115.
作者信息
Perepelov Andrei V, Xu Guangnan, Filatov Andrei V, Zhang Xinjie, Shashkov Alexander S, Wang Min, Knirel Yuriy A
机构信息
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, China; Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, 300071 Tianjin, China.
出版信息
Carbohydr Res. 2017 Aug 7;448:110-114. doi: 10.1016/j.carres.2017.05.021. Epub 2017 Jun 3.
An O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae C4115 and studied by sugar analysis along with 1D and 2D H and C NMR spectroscopy. The following structure of the linear pentasaccharide repeating unit of the O-polysaccharide was established: →2)-α-l-Rhap-(1 → 2)-α-l-Rhap-(1 → 2)-α-l-Rhap-(1 → 2)-α-d-Galp-(1 → 3)-α-d-FucpNAc-(1→ The O-antigen gene cluster of E. cloacae C4115 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in consistence with the O-polysaccharide structure. The O-antigen structure and gene cluster of E. cloacae C4115 are similar to those of E. cloacae G3421 studied by us earlier (Perepelov A.V. et al. Carbohydr. Res. 427 (2016) 55-59).
通过温和酸降解阴沟肠杆菌C4115的脂多糖分离得到一种O-多糖，并通过糖分析以及一维和二维氢谱与碳谱核磁共振波谱对其进行了研究。确定了该O-多糖线性五糖重复单元的以下结构：→2)-α-L-鼠李糖-(1→2)-α-L-鼠李糖-(1→2)-α-L-鼠李糖-(1→2)-α-D-半乳糖-(1→3)-α-D-岩藻糖胺-(1→ 对阴沟肠杆菌C4115的O抗原基因簇进行了测序。通过与现有数据库中的序列比较初步确定了基因功能，发现其与O-多糖结构一致。阴沟肠杆菌C4115的O抗原结构和基因簇与我们之前研究的阴沟肠杆菌G3421相似（佩列佩洛夫A.V.等人，《碳水化合物研究》427（2016）55-59）。
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