酿酒酵母中杀伤毒素KT28的细胞壁受体的分子结构。
Molecular structure of the cell wall receptor for killer toxin KT28 in Saccharomyces cerevisiae.
作者信息
Schmitt M, Radler F
机构信息
Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg-Universität Mainz, Federal Republic of Germany.
出版信息
J Bacteriol. 1988 May;170(5):2192-6. doi: 10.1128/jb.170.5.2192-2196.1988.
Abstract
The adsorption of the yeast killer toxin KT28 to susceptible cells of Saccharomyces cerevisiae was prevented by concanavalin A, which blocks the mannoprotein receptor. Certain mannoprotein mutants of S. cerevisiae that lack definite structures in the mannan of their cell walls were found to be resistant to KT28, whereas the wild-type yeast from which the mutants were derived was susceptible. Isolated mannoprotein from a resistant mutant was unable to adsorb killer toxin. By comparing the resistances of different mannoprotein mutants, information about the molecular structure of the receptor was obtained. At least two mannose residues have to be present in the side chains of the outer chain of the cell wall mannan, whereas the phosphodiester-linked mannose group is not essential for binding and the subsequent action of killer toxin KT28.
摘要
伴刀豆球蛋白A可阻止酵母杀伤毒素KT28吸附到酿酒酵母的敏感细胞上，因为它会阻断甘露糖蛋白受体。已发现酿酒酵母的某些甘露糖蛋白突变体，其细胞壁甘露聚糖中缺乏明确结构，对KT28具有抗性，而衍生出这些突变体的野生型酵母则敏感。从抗性突变体中分离出的甘露糖蛋白无法吸附杀伤毒素。通过比较不同甘露糖蛋白突变体的抗性，获得了有关受体分子结构的信息。细胞壁甘露聚糖外链的侧链中必须至少存在两个甘露糖残基，而磷酸二酯连接的甘露糖基团对于杀伤毒素KT28的结合及后续作用并非必需。
相似文献
1
Molecular structure of the cell wall receptor for killer toxin KT28 in Saccharomyces cerevisiae.酿酒酵母中杀伤毒素KT28的细胞壁受体的分子结构。
J Bacteriol. 1988 May;170(5):2192-6. doi: 10.1128/jb.170.5.2192-2196.1988.
2
Blockage of cell wall receptors for yeast killer toxin KT28 with antimannoprotein antibodies.用抗甘露糖蛋白抗体阻断酵母杀伤毒素KT28的细胞壁受体。
Antimicrob Agents Chemother. 1990 Aug;34(8):1615-8. doi: 10.1128/AAC.34.8.1615.
3
Killer-toxin-resistant kre12 mutants of Saccharomyces cerevisiae: genetic and biochemical evidence for a secondary K1 membrane receptor.酿酒酵母对杀伤毒素具有抗性的kre12突变体：二级K1膜受体的遗传学和生物化学证据
Arch Microbiol. 1995 Dec;164(6):435-43. doi: 10.1007/BF02529742.
4
Mannoprotein of the yeast cell wall as primary receptor for the killer toxin of Saccharomyces cerevisiae strain 28.酵母细胞壁的甘露糖蛋白作为酿酒酵母28菌株杀伤毒素的主要受体。
J Gen Microbiol. 1987 Dec;133(12):3347-54. doi: 10.1099/00221287-133-12-3347.
5
Kre1p, the plasma membrane receptor for the yeast K1 viral toxin.Kre1p，酵母K1病毒毒素的质膜受体。
Cell. 2002 Feb 8;108(3):395-405. doi: 10.1016/s0092-8674(02)00634-7.
6
A novel yeast gene, RHK1, is involved in the synthesis of the cell wall receptor for the HM-1 killer toxin that inhibits beta-1,3-glucan synthesis.一种新的酵母基因RHK1参与了HM-1杀伤毒素细胞壁受体的合成，该毒素可抑制β-1,3-葡聚糖的合成。
Mol Gen Genet. 1997 Mar 26;254(2):139-47. doi: 10.1007/s004380050401.
7
Isolation and characterization of Saccharomyces cerevisiae mutants with a different degree of resistance to killer toxins K1 and K2.对杀伤毒素K1和K2具有不同抗性程度的酿酒酵母突变体的分离与鉴定
FEMS Yeast Res. 2002 Mar;2(1):73-9. doi: 10.1111/j.1567-1364.2002.tb00070.x.
8
Killer toxin of Hanseniaspora uvarum.葡萄汁有孢汉逊酵母的杀伤毒素
Arch Microbiol. 1990;154(2):175-8. doi: 10.1007/BF00423329.
9
Different action of killer toxins K1 and K2 on the plasma membrane and the cell wall of Saccharomyces cerevisiae.杀伤毒素K1和K2对酿酒酵母质膜和细胞壁的不同作用。
FEMS Yeast Res. 2004 Sep;4(8):803-13. doi: 10.1016/j.femsyr.2004.04.007.
10
Electron microscopy of the K2 killer effect of Saccharomyces cerevisiae T206 on a mesophilic wine yeast.酿酒酵母T206对嗜温葡萄酒酵母的K2杀伤效应的电子显微镜观察
Antonie Van Leeuwenhoek. 2000 Aug;78(2):117-22. doi: 10.1023/a:1026588220367.
引用本文的文献
1
The Killer Toxin: From Origin to Biomedical Research.杀伤毒素：从起源到生物医学研究
Microorganisms. 2024 Dec 2;12(12):2481. doi: 10.3390/microorganisms12122481.
2
Expression of the K74 Killer Toxin from Saccharomyces paradoxus Is Modulated by the Toxin-Encoding M74 Double-Stranded RNA 5' Untranslated Terminal Region.来自奇异酵母的 K74 杀伤毒素的表达受毒素编码 M74 双链 RNA 5'非翻译末端区的调节。
Appl Environ Microbiol. 2022 Apr 26;88(8):e0203021. doi: 10.1128/aem.02030-21. Epub 2022 Apr 7.
3
Yeast Viral Killer Toxin K1 Induces Specific Host Cell Adaptions via Intrinsic Selection Pressure.酵母病毒杀手毒素 K1 通过内在选择压力诱导特定宿主细胞适应。
Appl Environ Microbiol. 2020 Feb 3;86(4). doi: 10.1128/AEM.02446-19.
4
Transcriptome Kinetics of in Response to Viral Killer Toxin K1.响应病毒杀伤性T毒素K1的转录组动力学
Front Microbiol. 2019 May 16;10:1102. doi: 10.3389/fmicb.2019.01102. eCollection 2019.
5
Production of fluorescent and cytotoxic K28 killer toxin variants through high cell density fermentation of recombinant Pichia pastoris.通过重组毕赤酵母高密度发酵生产荧光和细胞毒性 K28 杀伤毒素变体。
Microb Cell Fact. 2017 Dec 19;16(1):228. doi: 10.1186/s12934-017-0844-0.
6
Yeast Killer Toxin K28: Biology and Unique Strategy of Host Cell Intoxication and Killing.酵母杀伤毒素 K28：宿主细胞中毒和杀伤的生物学及独特策略。
Toxins (Basel). 2017 Oct 20;9(10):333. doi: 10.3390/toxins9100333.
7
The Biology of Pichia membranifaciens Killer Toxins.膜醭毕赤酵母杀伤毒素的生物学
Toxins (Basel). 2017 Mar 23;9(4):112. doi: 10.3390/toxins9040112.
8
H/KDEL receptors mediate host cell intoxication by a viral A/B toxin in yeast.H/KDEL受体介导酵母中病毒A/B毒素对宿主细胞的毒害作用。
Sci Rep. 2016 Aug 5;6:31105. doi: 10.1038/srep31105.
9
Yeast endocytic adaptor AP-2 binds the stress sensor Mid2 and functions in polarized cell responses.酵母内吞衔接蛋白 AP-2 结合应激传感器 Mid2 并在极化细胞反应中发挥作用。
Traffic. 2014 May;15(5):546-57. doi: 10.1111/tra.12155. Epub 2014 Feb 25.
10
A yeast killer toxin screen provides insights into a/b toxin entry, trafficking, and killing mechanisms.酵母杀伤毒素筛选为了解a/b毒素的进入、运输和杀伤机制提供了见解。
Dev Cell. 2009 Oct;17(4):552-60. doi: 10.1016/j.devcel.2009.08.006.
本文引用的文献
1
Construction of killer wine yeast strain.构建杀香醇酵母菌株。
Appl Environ Microbiol. 1985 May;49(5):1211-5. doi: 10.1128/aem.49.5.1211-1215.1985.
2
Physiology of killer factor in yeast.酵母中杀伤因子的生理学
Adv Microb Physiol. 1981;22:93-122. doi: 10.1016/s0065-2911(08)60326-4.
3
Saccharomyces cerevisiae mannoprotein mutants. Isolation of the mnn5 mutant and comparison with the mnn3 strain.酿酒酵母甘露糖蛋白突变体。mnn5突变体的分离及与mnn3菌株的比较。
J Biol Chem. 1980 Aug 25;255(16):7700-7.
4
Comparison of the killer toxin of several yeasts and the purification of a toxin of type K2.几种酵母杀伤毒素的比较及K2型毒素的纯化
Arch Microbiol. 1984 Apr;137(4):357-61. doi: 10.1007/BF00410734.
5
Carbohydrate structure of Saccharomyces cerevisiae mnn9 mannoprotein.酿酒酵母mnn9甘露糖蛋白的碳水化合物结构
J Biol Chem. 1984 Mar 25;259(6):3805-11.
6
Cell wall receptor for yeast killer toxin: involvement of (1 leads to 6)-beta-D-glucan.酵母杀伤毒素的细胞壁受体：（1→6）-β-D-葡聚糖的参与
J Bacteriol. 1983 Apr;154(1):161-9. doi: 10.1128/jb.154.1.161-169.1983.
7
The inheritance of the killer character in yeast.酵母中杀伤特性的遗传
Genet Res. 1969 Feb;13(1):71-83. doi: 10.1017/s0016672300002743.
8
Structure of the linkage region between the polysaccharide and protein parts of Saccharomyces cerevisiae mannan.酿酒酵母甘露聚糖多糖部分与蛋白质部分之间连接区域的结构
J Biol Chem. 1974 Dec 10;249(23):7685-94.
9
Characterization of the carbohydrate fragments obtained from Saccharomyces cerevisiae mannan by alkaline degradation.通过碱性降解对酿酒酵母甘露聚糖所得碳水化合物片段的表征。
J Biol Chem. 1974 Dec 10;249(23):7679-84.
10
Action of yeast killer factor: a resistant mutant with sensitive spheroplasts.酵母杀手因子的作用：具有敏感原生质球的抗性突变体。
J Bacteriol. 1973 Mar;113(3):1193-7. doi: 10.1128/jb.113.3.1193-1197.1973.
Zotero 插件