Dalvi Rishikesh S, Das Tilak, Debnath Dipesh, Yengkokpam Sona, Baruah Kartik, Tiwari Lalchand R, Pal Asim K
Division of Fish Nutrition and Biochemistry, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India; Department of Zoology, Maharshi Dayanand College, Parel, Mumbai 400012, India.
Division of Fish Nutrition and Biochemistry, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India; Department of Zoology, Raja N. L. Khan Womens' College, Paschim Medinipur 721102, West Bengal, India.
J Therm Biol. 2017 Apr;65:32-40. doi: 10.1016/j.jtherbio.2017.02.003. Epub 2017 Feb 7.
We investigated the metabolic and cellular stress responses in an endemic catfish Horabagrus brachysoma acclimated to ambient (26°C), 31, 33 and 36°C for 30 days. After acclimation, fish were sampled to investigate changes in the levels of blood glucose, tissue glycogen and ascorbic acid, activities of enzymes involved in glycolysis (LDH), citric acid cycle (MDH), gluconeogenesis (FBPase and G6Pase), pentose phosphate pathway (G6PDH), protein metabolism (AST and ALT), phosphate metabolism (ACP and ALP) and energy metabolism (ATPase), and HSP70 levels in various tissues. Acclimation to higher temperatures (33 and 36°C) significantly increased activities of LDH, MDH, ALP, ACP, AST, ALT and ATPase and blood glucose levels, whereas decreased the G6PDH enzyme activity and, tissue glycogen and ascorbic acid. Results indicated an overall increase in the carbohydrate, protein and lipid metabolism implying increased metabolic demands for maintaining homeostasis in fish acclimated to higher temperatures (33 and 36°C). We observed tissue specific response of HSP70 in H. brachysoma, with significant increase in gill and liver at 33 and 36°C, and in brain and muscle at 36°C, enabling cellular protection at higher acclimation temperatures. In conclusion, H. brachysoma adjusted metabolic and cellular responses to withstand increased temperatures, however, these responses suggest that the fish was under stress at 33°C or higher temperature.
我们研究了一种本地鲶鱼——短体霍氏鲶（Horabagrus brachysoma）在适应环境温度（26°C）、31°C、33°C和36°C 30天后的代谢和细胞应激反应。适应后，对鱼进行采样，以研究血糖、组织糖原和抗坏血酸水平的变化，以及参与糖酵解（乳酸脱氢酶，LDH）、柠檬酸循环（苹果酸脱氢酶，MDH）、糖异生（果糖-1,6-二磷酸酶，FBPase和葡萄糖-6-磷酸酶，G6Pase）、磷酸戊糖途径（葡萄糖-6-磷酸脱氢酶，G6PDH）、蛋白质代谢（天冬氨酸转氨酶，AST和丙氨酸转氨酶，ALT）、磷酸盐代谢（酸性磷酸酶，ACP和碱性磷酸酶，ALP）和能量代谢（ATP酶）的酶活性，以及各种组织中热休克蛋白70（HSP70）的水平。适应较高温度（33°C和36°C）显著增加了LDH、MDH、ALP、ACP、AST、ALT和ATP酶的活性以及血糖水平，而降低了G6PDH酶活性、组织糖原和抗坏血酸水平。结果表明碳水化合物、蛋白质和脂质代谢总体增加，这意味着适应较高温度（33°C和36°C）的鱼为维持体内平衡而对代谢的需求增加。我们观察到短体霍氏鲶中HSP70的组织特异性反应，在33°C和36°C时鳃和肝脏中显著增加，在36°C时脑和肌肉中显著增加，从而在较高的适应温度下实现细胞保护。总之，短体霍氏鲶调整了代谢和细胞反应以承受温度升高，然而，这些反应表明该鱼在33°C或更高温度下处于应激状态。
