Shibata D, Steczko J, Dixon J E, Andrews P C, Hermodson M, Axelrod B
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
J Biol Chem. 1988 May 15;263(14):6816-21.
The nucleotide sequence of soybean lipoxygenase-2 cDNA has been determined, and the complete amino acid sequence of the enzyme has been deduced. Limited direct amino acid sequence data for lipoxygenase-2 protein support this assignment and exclude mRNA representing lipoxygenase-1 and -3. Lipoxygenase 2 has a molecular weight of 97,036 and contains 865 amino acid residues, in contrast to the isozymes, lipoxygenase-1 and -3, which are known to contain 838 and 859 amino acid residues, respectively. Despite significant differences in behavior between these three isozymes, the amino acid sequences of lipoxygenase-1 and -3 are 81 and 74% identical to lipoxygenase-2, respectively. A region of 40 amino acid residues containing a cluster of six histidines and two tyrosines, which is highly conserved in all three isozymes, is discussed as a possible iron-binding region.
大豆脂氧合酶-2 cDNA的核苷酸序列已被确定，并推导了该酶完整的氨基酸序列。脂氧合酶-2蛋白有限的直接氨基酸序列数据支持这一归属，并排除了代表脂氧合酶-1和-3的mRNA。脂氧合酶2的分子量为97,036，含有865个氨基酸残基，而异构酶脂氧合酶-1和-3分别已知含有838和859个氨基酸残基。尽管这三种同工酶在行为上存在显著差异，但脂氧合酶-1和-3的氨基酸序列分别与脂氧合酶-2有81%和74%的同一性。在所有三种同工酶中高度保守的一个包含六个组氨酸和两个酪氨酸簇的40个氨基酸残基区域，被认为是一个可能的铁结合区域。
