Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), P.O. CIMAP, Near Kukrail Picnic Spot, Lucknow, 226015, India.
Botany Division, CSIR-Central Drug Research Institute, Sector 10, Jankipuram, Lucknow, 226031, India.
Plant Mol Biol. 2015 Sep;89(1-2):173-86. doi: 10.1007/s11103-015-0361-7. Epub 2015 Aug 30.
The AP2/ERFs are one of the most important family of transcription factors which regulate multiple responses like stress, metabolism and development in plants. We isolated PsAP2 a novel AP2/ERF from Papaver somniferum which was highly upregulated in response to wounding followed by ethylene, methyl jasmonate and ABA treatment. PsAP2 showed specific binding with both DRE and GCC box elements and it was able to transactivate the reporter genes in yeast. PsAP2 overexpressing transgenic tobacco plants exhibited enhanced tolerance towards both abiotic and biotic stresses . Real time transcript expression analysis showed constitutive upregulation of tobacco Alternative oxidase1a and Myo-inositol-1-phosphate synthase in PsAP2 overexpressing tobacco plants. Further, PsAP2 showed interaction with NtAOX1a promoter in vitro, it also specifically activated the NtAOX1a promoter in yeast and tobacco BY2 cells. The silencing of PsAP2 using VIGS lead to significant reduction in the AOX1 level in P. somniferum. Taken together PsAP2 can directly bind and transcriptionally activate NtAOX1a and its overexpression in tobacco imparted increased tolerance towards both abiotic and biotic stress.
AP2/ERF 是一类重要的转录因子家族，其在植物的多种生理反应中发挥调控作用，如胁迫应答、代谢和发育等。我们从罂粟中分离出一个新型的 AP2/ERF 基因 PsAP2，该基因在受到创伤后，对乙烯、茉莉酸甲酯和脱落酸的处理反应表现出高度上调。PsAP2 表现出与 DRE 和 GCC 框元件的特异性结合，并能在酵母中转激活报告基因。过表达 PsAP2 的转基因烟草植物对非生物和生物胁迫表现出增强的耐受性。实时转录表达分析显示，过表达 PsAP2 的烟草植物中替代氧化酶 1a 和肌醇-1-磷酸合酶持续上调。此外，PsAP2 与 NtAOX1a 启动子在体外相互作用，也能在酵母和烟草 BY2 细胞中特异性激活 NtAOX1a 启动子。利用 VIGS 沉默 PsAP2 会导致罂粟中 AOX1 水平显著降低。综上所述，PsAP2 可以直接结合并转录激活 NtAOX1a，其在烟草中的过表达赋予了对非生物和生物胁迫的增强耐受性。
