Nguyen Van Bon, Nguyen Anh Dzung, Kuo Yao-Haur, Wang San-Lang
Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan.
Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam.
Int J Mol Sci. 2017 Mar 25;18(4):700. doi: 10.3390/ijms18040700.
Paenibacillus sp. TKU042, a bacterium isolated from Taiwanese soil, produced α-glucosidase inhibitors (aGIs) in the culture supernatant when commercial nutrient broth (NB) was used as the medium for fermentation. The supernatant of fermented NB (FNB) showed stronger inhibitory activities than acarbose, a commercial anti-diabetic drug. The IC50 and maximum α-glucosidase inhibitory activities (aGIA) of FNB and acarbose against α-glucosidase were 81 μg/mL, 92% and 1395 μg/mL, 63%, respectively. FNB was found to be strongly thermostable, retaining 95% of its relative activity, even after heating at 100 °C for 30 min. FNB was also stable at various pH values. Furthermore, FNB demonstrated antioxidant activity (IC50 = 2.23 mg/mL). In animal tests, FNB showed remarkable reductions in the plasma glucose of ICR (Institute of Cancer Research) mice at a concentration of 200 mg/kg. Combining FNB and acarbose enhanced the effect even more, with an added advantage of eliminating diarrhea. According to HPLC (High-performance liquid chromatography) fingerprinting, the Paenibacillus sp. TKU042 aGIs were not acarbose. All of the results suggest that Paenibacillus sp. TKU042 FNB could have potential use as a health food or to treat type 2 diabetes.
从台湾土壤中分离出的芽孢杆菌属菌株TKU042，在以商业营养肉汤（NB）作为发酵培养基时，会在培养上清液中产生α-葡萄糖苷酶抑制剂（aGIs）。发酵NB（FNB）的上清液显示出比市售抗糖尿病药物阿卡波糖更强的抑制活性。FNB和阿卡波糖对α-葡萄糖苷酶的IC50和最大α-葡萄糖苷酶抑制活性（aGIA）分别为81μg/mL、92%和1395μg/mL、63%。发现FNB具有很强的热稳定性，即使在100℃加热30分钟后仍保留其95%的相对活性。FNB在各种pH值下也很稳定。此外，FNB表现出抗氧化活性（IC50 = 2.23mg/mL）。在动物试验中，FNB在浓度为200mg/kg时，使ICR（癌症研究所）小鼠的血浆葡萄糖显著降低。将FNB和阿卡波糖联合使用效果更佳，还有消除腹泻的额外优势。根据高效液相色谱（HPLC）指纹图谱分析，芽孢杆菌属菌株TKU042的aGIs不是阿卡波糖。所有结果表明，芽孢杆菌属菌株TKU042的FNB可能有作为健康食品或用于治疗2型糖尿病的潜在用途。
