Takeshima Tomomi, Tada Yuya, Sakaguchi Norihito, Watari Fumio, Fugetsu Bunshi
Graduate School of Environmental Science, Hokkaido University, Hokkaido 060-0810, Japan.
Nissei Bio Co. Ltd., Hokkaido 061-1374, Japan.
Nanomaterials (Basel). 2015 Mar 3;5(1):284-297. doi: 10.3390/nano5010284.
Silver (Ag) nanoparticles were produced using DNA extracted from salmon milt as templates. Particles spherical in shape with an average diameter smaller than 10 nm were obtained. The nanoparticles consisted of Ag as the core with an outermost thin layer of DNA. The DNA/Ag hybrid nanoparticles were immobilized over the surface of cotton based fabrics and their antibacterial efficiency was evaluated using as the typical Gram-negative bacteria. The antibacterial experiments were performed according to the Antibacterial Standard of Japanese Association for the Functional Evaluation of Textiles. The fabrics modified with DNA/Ag nanoparticles showed a high enough inhibitory and killing efficiency against at a concentration of Ag ≥ 10 ppm.
以从鲑鱼精液中提取的DNA为模板制备了银（Ag）纳米颗粒。获得了平均直径小于10nm的球形颗粒。纳米颗粒以Ag为核心，最外层为一层薄薄的DNA。将DNA/Ag杂化纳米颗粒固定在棉织物表面，并以作为典型的革兰氏阴性菌来评估其抗菌效率。抗菌实验按照日本纺织品功能评价协会的抗菌标准进行。用DNA/Ag纳米颗粒改性的织物在Ag浓度≥10ppm时对显示出足够高的抑制和杀灭效率。
