Wu Baoyan, Hou Shihua, Miao Zhiying, Zhang Cong, Ji Yanhong
MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
School of Electronic and Information Engineering, South China University of Technology, Guangzhou 510640, China.
Nanomaterials (Basel). 2015 Sep 18;5(3):1544-1555. doi: 10.3390/nano5031544.
A novel amperometric glucose biosensor was fabricated by layer-by-layer self-assembly of gold nanorods (AuNRs) and glucose oxidase (GOD) onto single-walled carbon nanotubes (SWCNTs)-functionalized three-dimensional sol-gel matrix. A thiolated aqueous silica sol containing SWCNTs was first assembled on the surface of a cleaned Au electrode, and then the alternate self-assembly of AuNRs and GOD were repeated to assemble multilayer films of AuNRs-GOD onto SWCNTs-functionalized silica gel for optimizing the biosensor. Among the resulting glucose biosensors, the four layers of AuNRs-GOD-modified electrode showed the best performance. The sol-SWCNTs-(AuNRs- GOD)₄/Au biosensor exhibited a good linear range of 0.01-8 mM glucose, high sensitivity of 1.08 μA/mM, and fast amperometric response within 4 s. The good performance of the proposed glucose biosensor could be mainly attributed to the advantages of the three-dimensional sol-gel matrix and stereo self-assembly films, and the natural features of one-dimensional nanostructure SWCNTs and AuNRs. This study may provide a new facile way to fabricate the enzyme-based biosensor with high performance.
通过将金纳米棒（AuNRs）和葡萄糖氧化酶（GOD）逐层自组装到单壁碳纳米管（SWCNTs）功能化的三维溶胶-凝胶基质上，制备了一种新型的安培型葡萄糖生物传感器。首先将含有SWCNTs的硫醇化水性硅溶胶组装在清洁后的金电极表面，然后重复AuNRs和GOD的交替自组装，以在SWCNTs功能化硅胶上组装AuNRs-GOD多层膜，从而优化生物传感器。在所得的葡萄糖生物传感器中，四层AuNRs-GOD修饰电极表现出最佳性能。溶胶-SWCNTs-(AuNRs-GOD)₄/Au生物传感器显示出0.01-8 mM葡萄糖的良好线性范围、1.08 μA/mM的高灵敏度以及在4 s内的快速安培响应。所提出的葡萄糖生物传感器的良好性能主要归因于三维溶胶-凝胶基质和立体自组装膜的优势，以及一维纳米结构SWCNTs和AuNRs的天然特性。该研究可能为制备高性能的基于酶的生物传感器提供一种新的简便方法。
