Heitland Peter, Blohm Martin, Breuer Christian, Brinkert Florian, Achilles Eike Gert, Pukite Ieva, Köster Helmut Dietrich
Medical Laboratory Bremen, Haferwende 12, D-28357 Bremen, Germany.
University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg, Germany.
J Trace Elem Med Biol. 2017 May;41:36-40. doi: 10.1016/j.jtemb.2017.02.008. Epub 2017 Feb 16.
ICP-MS and HPLC-ICP-MS were applied for diagnosis and therapeutic monitoring in a severe intoxication with a liquid containing hexavalent chromium (Cr(VI)) and inorganic arsenic (iAs). In this rare case a liver transplantation of was considered as the only chance of survival. We developed and applied methods for the determination of Cr(VI) in erythrocytes and total chromium (Cr) and arsenic (As) in blood, plasma, urine and liver tissue by ICP-MS. Exposure to iAs was diagnosed by determination of iAs species and their metabolites in urine by anion exchange HPLC-ICP-MS. Three days after ingestion of the liquid the total Cr concentrations were 2180 and 1070μg/L in whole blood and plasma, respectively, and 4540μg/L Cr(VI) in erythrocytes. The arsenic concentration in blood was 206μg/L. The urinary As species concentrations were <0.5, 109, 115, 154 and 126μg/L for arsenobetaine, As(III), As(V), methylarsonate (V) and dimethylarsinate (V), respectively. Total Cr and As concentrations in the explanted liver were 11.7 and 0.9mg/kg, respectively. Further analytical results of this case study are tabulated and provide valuable data for physicians and toxicologists.
电感耦合等离子体质谱（ICP-MS）和高效液相色谱-电感耦合等离子体质谱（HPLC-ICP-MS）被应用于对一起因摄入含六价铬（Cr(VI)）和无机砷（iAs）的液体而导致的严重中毒事件进行诊断和治疗监测。在这个罕见病例中，肝脏移植被认为是唯一的存活机会。我们开发并应用了通过ICP-MS测定红细胞中Cr(VI)以及血液、血浆、尿液和肝脏组织中总铬（Cr）和砷（As）的方法。通过阴离子交换HPLC-ICP-MS测定尿液中iAs的种类及其代谢物来诊断iAs暴露情况。摄入该液体三天后，全血和血浆中的总Cr浓度分别为2180和1070μg/L，红细胞中的Cr(VI)浓度为4540μg/L。血液中的砷浓度为206μg/L。尿中砷甜菜碱、As(III)、As(V)、甲基砷酸（V）和二甲基砷酸（V）的浓度分别为<0.5、109、115、154和126μg/L。移植肝脏中的总Cr和As浓度分别为11.7和0.9mg/kg。该病例研究的进一步分析结果已制成表格，为医生和毒理学家提供了有价值的数据。
