细胞核α-连环蛋白通过β-连环蛋白和细胞核肌动蛋白介导DNA损伤反应。
Nuclear α-catenin mediates the DNA damage response via β-catenin and nuclear actin.
作者信息
Serebryannyy Leonid A, Yemelyanov Alex, Gottardi Cara J, de Lanerolle Primal
机构信息
Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL 60612, USA
Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
出版信息
J Cell Sci. 2017 May 15;130(10):1717-1729. doi: 10.1242/jcs.199893. Epub 2017 Mar 27.
Abstract
α-Catenin is an F-actin-binding protein widely recognized for its role in cell-cell adhesion. However, a growing body of literature indicates that α-catenin is also a nuclear protein. In this study, we show that α-catenin is able to modulate the sensitivity of cells to DNA damage and toxicity. Furthermore, nuclear α-catenin is actively recruited to sites of DNA damage. This recruitment occurs in a β-catenin-dependent manner and requires nuclear actin polymerization. These findings provide mechanistic insight into the WNT-mediated regulation of the DNA damage response and suggest a novel role for the α-catenin-β-catenin complex in the nucleus.
摘要
α-连环蛋白是一种F-肌动蛋白结合蛋白，因其在细胞间黏附中的作用而广为人知。然而，越来越多的文献表明，α-连环蛋白也是一种核蛋白。在本研究中，我们表明α-连环蛋白能够调节细胞对DNA损伤和毒性的敏感性。此外，核α-连环蛋白会被主动招募到DNA损伤位点。这种招募以β-连环蛋白依赖的方式发生，并且需要核肌动蛋白聚合。这些发现为WNT介导的DNA损伤反应调节提供了机制性见解，并提示了α-连环蛋白-β-连环蛋白复合物在细胞核中的新作用。
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