Zhang X L, Langford A, Becker J, Rabanus J P, Pohle H D, Reichart P, Gelderblom H
Institute of Stomatology, Beijing Medical University, P.R. China.
Virchows Arch A Pathol Anat Histopathol. 1988;412(6):533-42. doi: 10.1007/BF00844289.
Three cases of HL from the lateral border of the tongue of male homosexual AIDS patients were investigated by thin section electron microscopy. Keratinocytes contained condensed chromatin in their pyknotic nuclei and a few organelles in the oedematous cytoplasm. Chromatin was in close association to the nuclear membrane and showed a punched-out appearance. Particles typical of the herpes virus group were abundant in the upper two thirds of the epithelium in all three cases. Virus particles were seen frequently in the nuclei of the ballooned keratinocytes, but rarely in cells containing Candida albicans. Viral nucleocapsids were observed budding at the inner nuclear membrane, thereby acquiring the prospective viral envelope. Complete, enveloped virions were found in the endoplasmic reticulum and in the extracellular space. These virions were identified immunohistochemically as Epstein-Barr virus (EBV) using two monoclonal antibodies directed against EBV capsid and membrane antigen, respectively. Candida albicans was observed in the stratum corneum and in the upper layer of the stratum spinosum. Special cytoplasmic tubular structures arranged in parallel bundles were found in koilocytotic cells in addition to characteristic membrane structures composed of undulating convoluted membranes. Epithelial basement membranes were always intact.
对3例男性同性恋艾滋病患者舌外侧缘的霍奇金淋巴瘤（HL）病例进行了超薄切片电子显微镜检查。角质形成细胞的固缩核内含有凝聚的染色质，水肿的细胞质中有一些细胞器。染色质与核膜紧密相连，呈穿孔状外观。在所有3例病例的上皮上三分之二区域，疱疹病毒组典型的颗粒丰富。病毒颗粒在气球样变的角质形成细胞核中常见，但在含有白色念珠菌的细胞中少见。观察到病毒核衣壳在内核膜处出芽，从而获得预期的病毒包膜。在内质网和细胞外空间发现了完整的、有包膜的病毒粒子。使用分别针对EB病毒衣壳抗原和膜抗原的两种单克隆抗体，通过免疫组织化学方法将这些病毒粒子鉴定为EB病毒（EBV）。在角质层和棘层上层观察到白色念珠菌。除了由波浪状卷曲膜组成的特征性膜结构外，在挖空细胞中还发现了平行排列成束的特殊细胞质管状结构。上皮基底膜始终完整。
