Pourzaki Mojtaba, Homayoun Mansour, Sadeghi Saeed, Seghatoleslam Masoumeh, Hosseini Mahmoud, Ebrahimzadeh Bideskan Alireza
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Pharmacological Research Center of Medicinal Plants , School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Avicenna J Phytomed. 2017 Mar-Apr;7(2):116-128.
() as a medicinal plant has been pointed to have analgesic, hypnotic and anti-oxidant effects. In the current study, a possible preventive effect of the hydro-alcoholic extract of the plant on neuronal damages was examined in pentylenetetrazole (PTZ) rat model of seizure.
Forty male rats were divided into five main groups and treated by (1) saline, (2) PTZ: 100 mg/kg PTZ (i.p) and (3-5) 50, 100 and 200 mg/kg of hydro-alcoholic extract of during seven consecutive days before PTZ injection. After electrocorticography (ECoG), the brains were removed to use for histological examination.
All doses of the extract reduced duration, frequency and amplitude of the burst discharges while prolonged the latency of the seizure attacks (p<0.05, p<0.01, and p<0.001). Administration of all 3 doses of the extract significantly prevented from production of dark neurons (p<0.01, and p<0.001) and apoptotic cells (p<0.05, p<0.01, and p<0.001) in different areas of the hippocampus compared to PTZ group.
The results of this study allow us to conclude that , because of its antioxidant properties, prevents from neuronal damages in PTZ rat model of seizure.
（某植物）作为一种药用植物，已被指出具有镇痛、催眠和抗氧化作用。在本研究中，在戊四氮（PTZ）诱导的大鼠癫痫模型中，检测了该植物水醇提取物对神经元损伤的潜在预防作用。
40只雄性大鼠分为五个主要组，并分别接受以下处理：（1）生理盐水；（2）PTZ：100mg/kg PTZ（腹腔注射）；以及（3 - 5）在注射PTZ前连续七天分别给予50、100和200mg/kg的该植物水醇提取物。在进行脑电图（ECoG）记录后，取出大脑用于组织学检查。
所有剂量的提取物均降低了爆发性放电的持续时间、频率和幅度，同时延长了癫痫发作的潜伏期（p<0.05，p<0.01，p<0.001）。与PTZ组相比，所有三个剂量的提取物给药均显著预防了海马不同区域中暗神经元（p<0.01，p<0.001）和凋亡细胞（p<0.05，p<0.01，p<0.001）的产生。
本研究结果使我们得出结论，（某植物）因其抗氧化特性，可预防PTZ诱导的大鼠癫痫模型中的神经元损伤。
