National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Sci Rep. 2017 Mar 28;7:45308. doi: 10.1038/srep45308.
Interactions between the host and gut microbiota can affect gut metabolism. In this study, the individual performances of 252 hens were recorded to evaluate feed efficiency. Hens with contrasting feed efficiencies (14 birds per group) were selected to investigate their duodenal, cecal and fecal microbial composition by sequencing the 16S rRNA gene V4 region. The results showed that the microbial community in the cecum was quite different from those in the duodenum and feces. The highest biodiversity and all differentially abundant taxa between the different efficiency groups were observed in the cecal microbial community with false discovery rate (FDR) <0.05. Of these differentially abundant cecal microbes, Lactobacillus accounted for a greater proportion than the others. The abundances of Lactobacillus and Akkermansia were significantly higher while that of Faecalibacterium was lower (FDR < 0.05) in the better feed efficiency (BFE) group. Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis revealed that the functions relating to glycometabolism and amino acid metabolism were enriched in the cecal microbiota of the BFE group. These results indicated the prominent role of cecal microbiota in the feed efficiency of chickens and suggested plausible uses of Lactobacillus to improve the feed efficiency of host.
宿主与肠道微生物群的相互作用会影响肠道代谢。本研究记录了 252 只母鸡的个体表现，以评估饲料效率。选择具有不同饲料效率（每组 14 只鸡）的母鸡，通过测序 16S rRNA 基因 V4 区来研究其十二指肠、盲肠和粪便中的微生物组成。结果表明，盲肠中的微生物群落与十二指肠和粪便中的微生物群落有很大的不同。在盲肠微生物群落中，观察到最高的生物多样性和所有在不同效率组之间差异丰度的分类群，假发现率（FDR）<0.05。在这些差异丰度的盲肠微生物中，乳酸菌的比例大于其他菌。在更好的饲料效率（BFE）组中，乳酸菌和阿克曼氏菌的丰度显著较高（FDR<0.05），而粪杆菌的丰度较低。通过未观察状态重建进行群落系统发育分析（PICRUSt）表明，盲肠微生物群中与糖代谢和氨基酸代谢相关的功能得到了富集。这些结果表明盲肠微生物群在鸡的饲料效率中起着重要作用，并暗示了使用乳酸菌来提高宿主的饲料效率的可能性。
