Rajkumar U, Vinoth A, Reddy E Pradeep Kumar, Shanmugam M, Rao S V Rama
a ICAR-Directorate of Poultry Research , Hyderabad , Telangana , India.
Anim Biotechnol. 2018 Jan 2;29(1):20-25. doi: 10.1080/10495398.2017.1287712. Epub 2017 Mar 28.
The effects of supplementing the organic forms of selenium (Se), chromium (Cr), and zinc (Zn) on Hsp-70 mRNA expression and body weight in broiler chickens were evaluated. 200 chicks were equally distributed into stainless steel battery brooders at the rate of 5 birds per pen and reared under heat stress condition up to 42 day. The chicks were fed with three experimental diets supplemented with organic forms of Se (0.30 mg/kg), Cr (2 mg/kg), and Zn (40 mg/kg) during the starter and finisher phases and a control diet without any supplementation. On the 21st and 42nd day, 20 birds from each period were sacrificed and samples were collected for analysis. Organic Se, Cr, and Zn supplementation significantly (P < 0.05) reduced the expression of Hsp-70 mRNA levels. The Hsp-70 mRNA expression levels were significantly (P < 0.05) different between the tissues studied with spleen having the lowest expression level. Hsp-70 mRNA expression level was not affected by age of the birds. The study concluded that organic trace mineral (oTM) supplementation resulted in low Hsp-70 mRNA expression, indicating reduced heat stress in broilers.
评估了补充有机形式的硒（Se）、铬（Cr）和锌（Zn）对肉鸡热休克蛋白70（Hsp - 70）mRNA表达和体重的影响。200只雏鸡以每栏5只的比例均匀分布在不锈钢层叠式育雏器中，在热应激条件下饲养至42日龄。在雏鸡的育雏期和育肥期，给雏鸡饲喂三种分别添加有机形式的硒（0.30毫克/千克）、铬（2毫克/千克）和锌（40毫克/千克）的试验日粮，以及一种不添加任何物质的对照日粮。在第21天和第42天，每个时期宰杀20只鸡，并采集样本进行分析。补充有机硒、铬和锌显著（P < 0.05）降低了Hsp - 70 mRNA水平的表达。在所研究的组织中，Hsp - 70 mRNA表达水平存在显著差异（P < 0.05），脾脏的表达水平最低。Hsp - 70 mRNA表达水平不受鸡龄的影响。该研究得出结论，补充有机微量元素（oTM）可导致Hsp - 70 mRNA低表达，表明肉鸡的热应激减轻。
