Priest Nicholas D, Blimkie Melinda S J, Wyatt Heather, Bugden Michelle, Bannister Laura A, Gueguen Yann, Jourdain Jean-Rene, Klokov Dmitry
*Radiobiology and Health Branch, Canadian Nuclear Laboratories, Plant Road, Chalk River, Ontario, K0J 1J0, Canada; †Retired; ‡L'Insitute de Radioprotection et de Sûreté Nucléaire, Fontenay-aux-Roses, Paris, France. §Tritium can be incorporated into a wide range of biological macromolecules and OBT represents a collective term for such tritium-containing macromolecules. Thus, various definitions of OBT exist. Within this study, term OBT refers to the three tritiated amino acids as described in Materials and Methods.
Health Phys. 2017 May;112(5):439-444. doi: 10.1097/HP.0000000000000637.
The objective of this study was to compare the biokinetics of injected H-labeled light (HTO) and heavy (DTO) water in CBA/CaJ mice and to compare the organ distribution and/or body content of H administered by chronic ingestion for 1 mo to C57Bl/6J mice, as either H-labeled water or H-labeled amino acids (glycine, alanine and proline). HTO and DTO were administered to CBA/CaJ mice by single intraperitoneal injection and body retention was determined for up to 384 h post-injection. Tritium-labeled water or H-labeled amino acids were given to C57Bl/6J mice ad libitum for 30 d in drinking water. Body content and organ distribution of H during the period of administration and subsequent to administration was determined by liquid scintillation counting. No differences were found between the biokinetics of HTO and DTO, indicating that data generated using HTO can be used to help assess the consequences of H releases from heavy water reactors. The results for H-water showed that the concentration of radionuclide in the mice reached a peak after about 10 d and dropped rapidly after the cessation of H administration. The maximum concentration reached was only 50% of that in the water consumed, indicating that mice receive a significant fraction of their water from respiration. Contrary to the findings of others, the pattern of H retention following the administration of a cocktail of the labeled amino acids was very little different from that found for the water. This is consistent with the suggestion that most of the ingested amino acids were rapidly metabolized, releasing water and carbon dioxide.
本研究的目的是比较注射的氢标记轻水（HTO）和重水（DTO）在CBA/CaJ小鼠体内的生物动力学，并比较连续1个月慢性摄入氢给C57Bl/6J小鼠后，以氢标记水或氢标记氨基酸（甘氨酸、丙氨酸和脯氨酸）形式给予的氢在器官中的分布和/或体内含量。通过单次腹腔注射将HTO和DTO给予CBA/CaJ小鼠，并在注射后长达384小时测定体内滞留情况。将氚标记水或氢标记氨基酸随意添加到C57Bl/6J小鼠的饮用水中30天。通过液体闪烁计数法测定给药期间及给药后体内氢的含量和器官分布。未发现HTO和DTO的生物动力学存在差异，这表明使用HTO生成的数据可用于帮助评估重水反应堆中氢释放的后果。氢水的结果表明，小鼠体内放射性核素浓度在约10天后达到峰值，停止给予氢后迅速下降。达到的最大浓度仅为所消耗水中浓度的50%，这表明小鼠通过呼吸获取了相当一部分水分。与其他人的研究结果相反，给予标记氨基酸混合物后氢的滞留模式与给予水时发现的模式几乎没有差异。这与以下观点一致，即摄入的大多数氨基酸迅速代谢，释放出水和二氧化碳。
