Kopańska Marta, Szala Dariusz, Czech Joanna, Gabło Natalia, Gargasz Krzysztof, Trzeciak Mateusz, Zawlik Izabela, Snela Sławomir
Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, University of Rzeszow, Warzywna 1A, 35-959, Rzeszow, Poland.
Department of Genetics, Chair of Molecular Medicine, Faculty of Medicine, University of Rzeszow, Warzywna 1A, 35-959, Rzeszow, Poland.
J Orthop Surg Res. 2017 Mar 28;12(1):51. doi: 10.1186/s13018-017-0542-y.
Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA.
Forty tissue samples from 29 patients undergoing joint replacement for OA were evaluated. Tissue from two control patients undergoing hip replacement not related to OA was used as a control. Total RNA (containing miRNA species) from cartilage was isolated using a miRCURYTM [corrected] Isolation Kit. Expression of 19 miRNAs was assessed by real-time quantitative polymerase chain reaction.
Expression of four miRNAs, miR-138-5p, miR-146a-5p, miR-335-5p, and miR-9-5p, was significantly upregulated in OA tissues (patients vs. control group).
These findings may contribute to disease prevention and the development of therapeutic targets for OA.
骨关节炎（OA）是关节最常见的疾病，是一种由遗传、机械和环境因素引起的复杂多因素疾病。在本研究中，我们评估了OA中miRNA的表达。
对29例因OA接受关节置换术患者的40份组织样本进行评估。将2例接受与OA无关的髋关节置换术的对照患者的组织用作对照。使用miRCURYTM [校正后]分离试剂盒从软骨中分离总RNA（包含miRNA种类）。通过实时定量聚合酶链反应评估19种miRNA的表达。
在OA组织中（患者与对照组相比），四种miRNA，即miR-138-5p、miR-146a-5p、miR-335-5p和miR-9-5p的表达显著上调。
这些发现可能有助于OA的疾病预防和治疗靶点的开发。
