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植物线粒体DNA中环状分子的大小分布

Size distributions of circular molecules in plant mitochondrial DNAs.

作者信息

Bailey-Serres J, Leroy P, Jones S S, Wahleithner J A, Wolstenholme D R

机构信息

Department of Biology, University of Utah, Salt Lake City 84112.

出版信息

Curr Genet. 1987;12(1):49-53. doi: 10.1007/BF00420727.

DOI:10.1007/BF00420727
PMID:2835173
Abstract

Some physicochemical properties of the mitochondrial DNAs (mtDNA) from plants of flax, broad bean and mung bean, and from tissue culture cells of jimson weed, soybean, petunia and tobacco were determined. Circular molecules were observed in electron microscope preparations of each mtDNA. In soybean, petunia, broad bean and mung bean mtDNAs, the circular molecules had a continuous distribution of lengths (ranges between 1 to 36 kb, and 1 to 126 kb), heavily skewed toward smaller molecules. Eighty-six percent of the flax circular molecules were from 27 to 54 kb in size, and 78% of the jimson weed circular molecules were from 4 to 15 kb. Replicative forms of 1.2-1.6 kb circular molecules were observed in electron microscope preparations of broad bean mtDNA.

摘要

测定了亚麻、蚕豆和绿豆植株以及曼陀罗、大豆、矮牵牛和烟草组织培养细胞中线粒体DNA(mtDNA)的一些物理化学性质。在每个mtDNA的电子显微镜制备物中均观察到环状分子。在大豆、矮牵牛、蚕豆和绿豆的mtDNA中,环状分子的长度呈连续分布(范围在1至36 kb以及1至126 kb之间),严重偏向较小的分子。86%的亚麻环状分子大小在27至54 kb之间,78%的曼陀罗环状分子大小在4至15 kb之间。在蚕豆mtDNA的电子显微镜制备物中观察到了1.2 - 1.6 kb环状分子的复制形式。

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Circular plasmid DNAs from mitochondria of Sorghum bicolor.高粱线粒体中的环状质粒 DNA。
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Comparative electrophoretical analysis of plasmid-like mitochondrial DNAs in Vicia faba and in some other legumes.菜豆属和其它一些豆类的类线粒体质粒 DNA 的比较电泳分析。
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