Huang Yuxin, Wang Haidong, Li Yuan, Tao Xiaoming, Sun Jiao
Department of Endocrinology, Huadong Hospital Affiliated to Fudan University, 221 Yananxi Road, Shanghai 200040, China; Shanghai Key Laboratory of Clinical Geriatric Medicine, Shanghai, China.
Int J Endocrinol. 2017;2017:4578973. doi: 10.1155/2017/4578973. Epub 2017 Mar 2.
. We investigated whether poor sleep quality is associated with both dawn phenomenon and impaired circadian clock gene expression in subjects with diabetes. . 81 subjects with diabetes on continuous glucose monitoring were divided into two groups according to the Pittsburgh Sleep Quality Index. The magnitude of dawn phenomenon was quantified by its increment from nocturnal nadir to prebreakfast. Peripheral leucocytes were sampled from 81 subjects with diabetes and 28 normal controls at 09:00. Transcript levels of circadian clock genes (, , , and ) were determined by real-time quantitative polymerase chain reaction. The levels of HbA1c and fasting glucose and the magnitude of dawn phenomenon were significantly higher in the diabetes group with poor sleep quality than that with good sleep quality. Peripheral leucocytes from subjects with poor sleep quality expressed significantly lower transcript levels of and compared with those with good sleep quality. Poor sleep quality was significantly correlated with magnitude of dawn phenomenon. Multiple linear regression showed that sleep quality and were significantly independently correlated with dawn phenomenon. . Dawn phenomenon is associated with sleep quality. Furthermore, mRNA expression of circadian clock genes is dampened in peripheral leucocytes of subjects with poor sleep quality.
我们调查了睡眠质量差是否与糖尿病患者的黎明现象和昼夜节律钟基因表达受损有关。对81名进行连续血糖监测的糖尿病患者，根据匹兹堡睡眠质量指数分为两组。黎明现象的程度通过其从夜间最低点到早餐前的增加值来量化。在09:00从81名糖尿病患者和28名正常对照中采集外周血白细胞。通过实时定量聚合酶链反应测定昼夜节律钟基因（、、、和）的转录水平。睡眠质量差的糖尿病组的糖化血红蛋白、空腹血糖水平和黎明现象的程度显著高于睡眠质量好的糖尿病组。与睡眠质量好的受试者相比，睡眠质量差的受试者外周血白细胞中、的转录水平显著降低。睡眠质量差与黎明现象的程度显著相关。多元线性回归显示，睡眠质量和与黎明现象显著独立相关。黎明现象与睡眠质量有关。此外，睡眠质量差的受试者外周血白细胞中昼夜节律钟基因的mRNA表达受到抑制。
