酿酒酵母减数分裂和有丝分裂基因转换片段的物理长度。
Physical lengths of meiotic and mitotic gene conversion tracts in Saccharomyces cerevisiae.
作者信息
Judd S R, Petes T D
机构信息
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
出版信息
Genetics. 1988 Mar;118(3):401-10. doi: 10.1093/genetics/118.3.401.
Abstract
Physical lengths of gene conversion tracts for meiotic and mitotic conversions were examined, using the same diploid yeast strain in all experiments. This strain is heterozygous for a mutation in the URA3 gene as well as closely linked restriction site markers. In cells that had a gene conversion event at the URA3 locus, it was determined by Southern analysis which of the flanking heterozygous restriction sites had co-converted. It was found that mitotic conversion tracts were longer on the average than meiotic tracts. About half of the tracts generated by spontaneous mitotic gene conversion included heterozygous markers 4.2 kb apart; none of the meiotic conversions included these markers. Stimulation of mitotic gene conversion by ultraviolet light or methylmethanesulfonate had no obvious effect on the size or distribution of the tracts. Almost all conversion tracts were continuous.
摘要
利用同一二倍体酵母菌株，在所有实验中检测了减数分裂和有丝分裂基因转换片段的物理长度。该菌株在URA3基因以及紧密连锁的限制性位点标记上是杂合的。在URA3位点发生基因转换事件的细胞中，通过Southern分析确定了侧翼杂合限制性位点中的哪一个发生了共转换。结果发现，有丝分裂转换片段平均比减数分裂片段长。自发有丝分裂基因转换产生的片段中，约一半包含相距4.2 kb的杂合标记；减数分裂转换中没有一个包含这些标记。紫外线或甲基磺酸甲酯对有丝分裂基因转换的刺激对片段的大小或分布没有明显影响。几乎所有的转换片段都是连续的。
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