Garousi Farzaneh, Domokos-Szabolcsy Éva, Jánószky Mihály, Kovács Andrea Balláné, Veres Szilvia, Soós Áron, Kovács Béla
Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Food Science, University of Debrecen, Böszörményi str. 138, Debrecen, H-4032, Hungary.
Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Crop Sciences, Department of Agricultural Botany, Crop Physiology and Biotechnology, University of Debrecen, Böszörményi str. 138, Debrecen, H-4032, Hungary.
Plant Foods Hum Nutr. 2017 Jun;72(2):168-175. doi: 10.1007/s11130-017-0606-5.
Selenium deficiency in various degrees affects around 15% of the world's population, contributing to a variety of health problems. In this study, we examined the accumulation and biotransformation of soil applied Se-supplementation (sodium selenite and sodium selenate forms) at different concentrations, along with growth and yield formation of green pea, in a greenhouse experiment. Biotransformation of inorganic Se was evaluated using HPLC-ICP-MS for Se-species separation in the above ground parts of green pea. Results showed 3 mg kg Se increased green pea growth biomarkers and also caused an increase in protein content in leaves by 17%. Selenomethionine represented 65% of the total selenium content in shoots, but was lower in pods and seeds (54 and 38%, respectively). Selenomethionine was the major species in all plant parts and the only organic selenium form in the lower Se concentration range. Elevating the dose of Se (≥30 mg kg) triggered detrimental effects on growth and protein content and caused higher accumulation of inorganic Se in forms of Se and Se. Selenocysteine, another organic form of proteinogenic amino acid, was determined when Se (≥10 mg kg) was applied in higher concentrations. Thus, agronomic biofortification using the appropriate chemical form and concentration of Se will have positive effects on green pea growth and its enriched shoots and seeds provide a value-added protein source for livestock and humans with significant increased selenomethionine.
不同程度的硒缺乏影响着全球约15%的人口，导致各种健康问题。在本研究中，我们在温室试验中研究了不同浓度土壤施用硒补充剂（亚硒酸钠和硒酸钠形式）的积累和生物转化，以及青豆的生长和产量形成。使用HPLC-ICP-MS评估无机硒在青豆地上部分的生物转化，以分离硒形态。结果表明，3 mg/kg的硒增加了青豆生长生物标志物，还使叶片中的蛋白质含量增加了17%。硒代蛋氨酸占地上部分总硒含量的65%，但在豆荚和种子中较低（分别为54%和38%）。硒代蛋氨酸是所有植物部位的主要形态，也是低硒浓度范围内唯一的有机硒形态。提高硒剂量（≥30 mg/kg）对生长和蛋白质含量产生有害影响，并导致以硒酸盐和亚硒酸盐形式存在的无机硒积累增加。当以较高浓度施用硒（≥10 mg/kg）时，还检测到另一种蛋白质ogenic氨基酸的有机形态——硒代半胱氨酸。因此，使用适当化学形态和浓度的硒进行农艺生物强化将对青豆生长产生积极影响，其富含硒的地上部分和种子为牲畜和人类提供了增值蛋白质来源，且硒代蛋氨酸含量显著增加。
