Heberling R L, Kalter S S, Smith J S, Hildebrand D G
J Clin Microbiol. 1987 Jul;25(7):1262-4. doi: 10.1128/jcm.25.7.1262-1264.1987.
A dot immunobinding assay that uses inactivated antigen for the detection of rabies viral antibodies was compared with the rapid fluorescent focus inhibition test. Results of testing pre- and postvaccination sera from humans (n = 33) and canines (n = 22) were identical for both tests. Endpoint titers of positive sera also were approximately the same by both methods. When a mouse monoclonal antibody was used, the dot immunobinding assay antigen was shown to possess detectable rabies virus glycoprotein and core antigens.
将一种使用灭活抗原检测狂犬病病毒抗体的斑点免疫结合试验与快速荧光灶抑制试验进行了比较。对人类(n = 33)和犬类(n = 22)接种疫苗前后的血清进行检测,两种试验的结果相同。两种方法检测阳性血清的终点滴度也大致相同。当使用小鼠单克隆抗体时,斑点免疫结合试验抗原显示具有可检测到的狂犬病病毒糖蛋白和核心抗原。