Wu Bao-Qiang, Jiang Yong, Zhu Feng, Sun Dong-Lin, He Xiao-Zhou
Department of Hepatatobiliary Surgery, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu Province, China.
Technol Cancer Res Treat. 2017 Dec;16(6):819-827. doi: 10.1177/1533034617700559. Epub 2017 Mar 30.
Long noncoding RNA-plasmacytoma variant translocation 1 is identified to be highly expressed and exhibits oncogenic activity in a variety of human malignancies, including pancreatic cancer. However, little is known about the overall biological role and mechanism of plasmacytoma variant translocation 1 in pancreatic cancer so far. In this study, we investigated the effect of plasmacytoma variant translocation 1 on pancreatic cancer cell proliferation and migration as well as epithelial-mesenchymal transition.
Pancreatic cancer tissue specimens and cell line were used in this study, with normal tissue and cell line acting as control.
It showed that plasmacytoma variant translocation 1 expression was significantly upregulated in pancreatic cancer tissues or cell line compared to normal groups. Plasmacytoma variant translocation 1 downregulation significantly inhibited zinc finger E-box-binding protein 1/Snail expression but promoted p21 expression, and it also inhibited the cell proliferation and migration. Additionally, p21 downregulation enhanced, and p21 overexpression repressed, zinc finger E-box-binding protein 1/Snail expression and cells proliferation in PANC-1 cells. However, p21 downregulation reversed the effect of plasmacytoma variant translocation 1 downregulation on zinc finger E-box-binding protein 1/Snail expression and cell proliferation and migration.
Plasmacytoma variant translocation 1 promoted epithelial-mesenchymal transition and cell proliferation and migration through downregulating p21 in pancreatic cancer cells.
长链非编码RNA-浆细胞瘤变异易位1被证实高表达，并在包括胰腺癌在内的多种人类恶性肿瘤中表现出致癌活性。然而，目前对于浆细胞瘤变异易位1在胰腺癌中的整体生物学作用及机制知之甚少。在本研究中，我们探究了浆细胞瘤变异易位1对胰腺癌细胞增殖、迁移以及上皮-间质转化的影响。
本研究使用了胰腺癌组织标本和细胞系，以正常组织和细胞系作为对照。
结果显示，与正常组相比，浆细胞瘤变异易位1在胰腺癌组织或细胞系中的表达显著上调。浆细胞瘤变异易位1表达下调显著抑制锌指E盒结合蛋白1/蜗牛蛋白的表达，但促进p21的表达，同时也抑制细胞增殖和迁移。此外，p21表达下调增强，而p21过表达则抑制PANC-1细胞中锌指E盒结合蛋白1/蜗牛蛋白的表达及细胞增殖。然而，p21表达下调逆转了浆细胞瘤变异易位1表达下调对锌指E盒结合蛋白1/蜗牛蛋白表达以及细胞增殖和迁移的影响。
浆细胞瘤变异易位1通过下调胰腺癌细胞中的p21促进上皮-间质转化以及细胞增殖和迁移。
