Fukuda R, Nishimura A, Serizawa H
Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.
Mol Gen Genet. 1988 Mar;211(3):515-9. doi: 10.1007/BF00425709.
To determine its map position, the sSP gene was cloned into plasmid pBR322 and the recombinant plasmid was integrated into the chromosome of a polA mutant at the site of the sSP gene by homologous recombination. The chromosomal location of Ampr was then determined by P1 phage-mediated transduction. Thus, the sSP gene was mapped between gltB and glnF at min 69.5 on the Escherichia coli chromosome. Strains were constructed in which the sSP gene was brought under the control of the lac regulatory system. This indicated that the stringent starvation protein (SSP) is dispensable for growth, at least under normal culture conditions.
为了确定其图谱位置，将sSP基因克隆到质粒pBR322中，然后通过同源重组将重组质粒整合到polA突变体染色体上sSP基因的位点。随后通过P1噬菌体介导的转导确定Ampr的染色体定位。因此，sSP基因被定位在大肠杆菌染色体上69.5分钟处的gltB和glnF之间。构建了使sSP基因受lac调控系统控制的菌株。这表明，至少在正常培养条件下，严格饥饿蛋白（SSP）对于生长是可有可无的。
