El-Kheshen Gadier, Moeini Maryam, Saadat Mostafa
Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
J Med Biochem. 2016 Sep;35(3):333-336. doi: 10.1515/jomb-2016-0002. Epub 2016 Jul 6.
Reactive oxygen species can attack and damage almost every molecule found in living cells, including proteins, carbohydrates, lipids, and DNA. For this reason, their production is normally tightly controlled. Among the most important defenses against these radicals are the superoxide dismutase (SOD) enzymes and catalase (CAT). Increasing attention has been given to the role of reactive oxygen metabolites in the pathogenesis of ulcerative colitis (UC), which is defined as an idiopathic and chronic intestinal inflammation. Accordingly, we hypothesized a relation between genetic polymorphisms in the two antioxidant enzymes A251G (rs2070424) and C-262T (rs1001179) and the risk of UC.
The present case-control study included 109 UC patients (46 males and 50 females) and 186 (67 males and 119 females) gender-matched healthy controls. Genotyping was done by the PCR-RFLP method.
After adjusting for age and gender, a significant association was observed between the AG+GG genotypes of A251G polymorphism (vs. AA genotype) and risk of UC (OR=0.29, 95% CI: 0.10-0.86, P= 0.025) after adjusting for age and gender. Our statistical analysis revealed that the C-262T polymorphism did not associate with the risk of UC before and/or after adjusting for age and gender.
Based on the present statistical analysis, the G allele of the A251G polymorphism decreases the risk of UC, thus it might be assumed that the G allele has a protective role.
活性氧可攻击并损害活细胞中几乎所有的分子，包括蛋白质、碳水化合物、脂质和DNA。因此，其生成通常受到严格控制。对抗这些自由基的最重要防御机制之一是超氧化物歧化酶（SOD）和过氧化氢酶（CAT）。活性氧代谢产物在溃疡性结肠炎（UC）发病机制中的作用受到了越来越多的关注，UC被定义为一种特发性慢性肠道炎症。因此，我们推测两种抗氧化酶A251G（rs2070424）和C-262T（rs1001179）的基因多态性与UC风险之间存在关联。
本病例对照研究纳入了109例UC患者（46例男性和50例女性）以及186例（67例男性和119例女性）性别匹配的健康对照。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行基因分型。
在调整年龄和性别后，A251G多态性的AG + GG基因型（与AA基因型相比）与UC风险之间存在显著关联（OR = 0.29，95%可信区间：0.10 - 0.86，P = 0.025）。我们的统计分析显示，C-262T多态性在调整年龄和性别之前和/或之后与UC风险均无关联。
基于目前的统计分析，A251G多态性的G等位基因可降低UC风险，因此可以推测G等位基因具有保护作用。
