首次对来自中东地区的一种蜜蜂毒液进行的描述。
First Characterization of The Venom from , A Honeybee from The Middle East Region.
机构信息
Laboratory of Applied Biotechnology (LBA3B), Azm Center for Research in Biotechnology and its Applications, EDST, Lebanese University, Tripoli 1300, Lebanon.
Faculty of Sciences 3, Lebanese University, Michel Slayman Tripoli Campus, Ras Maska 1352, Lebanon.
出版信息
Toxins (Basel). 2019 Mar 30;11(4):191. doi: 10.3390/toxins11040191.
Bee venom is a mixture of several components with proven therapeutic benefits, among which are anti-inflammatory, analgesic, and various cardiovascular conditions. In this work, we analyzed for the first time the proteomic content and biological properties of the crude venom from , a honeybee from the Middle East region. Using high-performance liquid chromatography-tandem mass spectrometry, we evidence the venom contains phospholipase A2, hyaluronidase, mast cell-degranulating peptide, adolapin, apamin, and melittin. The latter was purified by solid phase extraction method (SPE) and tested in parallel with crude venom for biological activities. Precisely, crude venom-but not melittin-exhibited antibacterial activity against and strains. Alongside, hemolytic activity was observed in human blood subjected to the venom at high doses. venom displayed antioxidant activities, and not surprisingly, PLA2 catalytic activity. Eventually, the venom proved to exert antiproliferative effects against MCF-7 and 3T3 cancer cells lines. This first report of a new bee venom opens new avenues for therapeutic uses of bee venoms.
蜂毒是一种含有多种成分的混合物，具有已被证实的治疗益处，包括抗炎、镇痛和各种心血管疾病。在这项工作中，我们首次分析了来自中东地区的一种蜜蜂的粗蜂毒的蛋白质组内容和生物学特性。我们使用高效液相色谱-串联质谱法证明，蜂毒含有磷脂酶 A2、透明质酸酶、肥大细胞脱粒肽、adolapin、apamin 和 melittin。后者通过固相萃取法 (SPE) 进行纯化，并与粗蜂毒进行平行测试以评估其生物学活性。具体来说，粗蜂毒而非 melittin 对 和 菌株表现出抗菌活性。此外，在高剂量下用人血进行实验时观察到溶血活性。 蜂毒具有抗氧化活性，而 PLA2 催化活性则不出所料。最终，蜂毒对 MCF-7 和 3T3 癌细胞系表现出抗增殖作用。这是对一种新蜂毒的首次报道，为蜂毒的治疗用途开辟了新途径。
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