Rinchai Darawan, Anguiano Esperanza, Nguyen Phuong, Chaussabel Damien
Systems Biology Department, Sidra Medical and Research Center, Doha, Qatar.
The Jackson Laboratory for Genomic Medicine, Farmington, USA.
F1000Res. 2016 Jun 15;5:1385. doi: 10.12688/f1000research.8841.2. eCollection 2016.
With this report we aim to make available a standard operating procedure (SOP) developed for RNA stabilization of small blood volumes collected via a finger stick. The anticipation that this procedure may be improved through peer-review and/or readers public comments is another element motivating the publication of this SOP. Procuring blood samples from human subjects can, among other uses, enable assessment of the immune status of an individual subject via the profiling of RNA abundance using technologies such as real time PCR, NanoString, microarrays or RNA-sequencing. It is often desirable to minimize blood volumes and employ methods that are the least invasive and can be practically implemented outside of clinical settings. Finger stick blood samples are increasingly used for measurement of levels of pharmacological drugs and biological analytes. It is a simple and convenient procedure amenable for instance to field use or self-collection at home using a blood sample collection kit. Such methodologies should also enable the procurement of blood samples at high frequency for health or disease monitoring applications.
通过本报告，我们旨在提供一种为经手指采血收集的少量血液的RNA稳定化而制定的标准操作程序（SOP）。预期该程序可通过同行评审和/或读者的公众意见得到改进，这是推动本SOP发表的另一个因素。从人类受试者获取血液样本，除其他用途外，可通过使用实时PCR、NanoString、微阵列或RNA测序等技术分析RNA丰度，来评估个体受试者的免疫状态。通常希望尽量减少采血量，并采用侵入性最小且可在临床环境之外实际实施的方法。手指采血样本越来越多地用于测量药物和生物分析物的水平。这是一种简单方便的程序，例如适用于现场使用或在家中使用血液样本采集试剂盒自行采集。此类方法还应能够高频采集血液样本，用于健康或疾病监测应用。
