Badr M, Marchetti B, Pelletier G
MRC Group in Molecular Endocrinology, Le Centre Hospitalier de l'Université Laval, Quebec, Canada.
Peptides. 1988 Mar-Apr;9(2):441-2. doi: 10.1016/0196-9781(88)90283-5.
The modulation of brain LHRH receptors by sex steroids was assessed in the female rat hippocampus using in vitro autoradiography and iodinated [D-Ser (TBU)6, des-Gly-NH2(10)]LHRH ethylamide as the radioligand. As evaluated by optical densitometry, the density of hippocampal LHRH receptors was increased by castration. In castrated animals, estradiol administration produced a small decrease in receptor concentration, whereas the concomitant administration of progesterone and estradiol as well as the injection of dihydrotestosterone induced a marked decrease in receptor density. Progesterone administration did not produce any significant change in receptor concentration. These results indicate that brain LHRH receptors can be modulated by sex steroids. Interestingly, this modulation of hippocampal LHRH receptors is similar to that previously observed in pituitary gland.
利用体外放射自显影技术，并以碘化的[D-丝氨酸（叔丁基）6，去甘氨酰胺（10）]促性腺激素释放激素乙酰胺作为放射性配体，在雌性大鼠海马中评估了性类固醇对脑促性腺激素释放激素（LHRH）受体的调节作用。通过光密度测定法评估，去势可增加海马LHRH受体的密度。在去势动物中，给予雌二醇会使受体浓度略有下降，而同时给予孕酮和雌二醇以及注射双氢睾酮会导致受体密度显著下降。给予孕酮不会使受体浓度产生任何显著变化。这些结果表明，脑LHRH受体可受到性类固醇的调节。有趣的是，海马LHRH受体的这种调节作用与先前在垂体中观察到的相似。
