Xu Yixiang, Wang Chao, Zhang Gang, Tian Jingjing, Liu Ying, Shen Xihui, Feng Jie
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Arch Microbiol. 2017 Aug;199(6):891-896. doi: 10.1007/s00203-017-1365-2. Epub 2017 Mar 29.
58 multiresistant strains representing diverse genera were isolated from farmed fish in an aquaculture facility. Resistant rates of strains harboring ISCR2, an insertion sequence type element, were higher than those in which this element was absent. Full genome sequencing of a Vibrio isolate containing ISCR2 confirmed that it is associated with multiple resistance genes, many of which are of clinical relevance. We describe the structural variation within ISCR2, and its distribution throughout multiple diverse aquatic genera, including Vibrio, Shewenalla, Pseudoalteromonas and Psychrobacter, suggesting the potential role of ISCR2 in disseminating antibiotic resistance. We also observe, and experimentally verify, a novel macrolide resistance gene that is also associated with ISCR2.
从一家水产养殖设施的养殖鱼类中分离出58株代表不同属的多重耐药菌株。携带ISCR2（一种插入序列类型元件）的菌株的耐药率高于不含该元件的菌株。对一株含有ISCR2的弧菌分离株进行全基因组测序证实，它与多个耐药基因相关，其中许多基因具有临床相关性。我们描述了ISCR2内的结构变异，及其在多个不同水生属（包括弧菌属、希瓦氏菌属、假交替单胞菌属和嗜冷杆菌属）中的分布，表明ISCR2在传播抗生素耐药性方面的潜在作用。我们还观察到并通过实验验证了一个也与ISCR2相关的新型大环内酯耐药基因。
