Abdelaziz Gobarah Dalia Elsayed, Helmy Salwa Mahmoud, Mahfouz Nadia Bassiony, Fahmy Hanan Ali, Abou Zeid Mayada Abd El Hamied Muhammed
Department of Bacteriology, Animal Health Research Institute, Kafr El -Sheikh Branch, Agriculture Research Center, Giza, Egypt.
Department of Microbiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr EL-Sheikh, Egypt.
Vet Res Forum. 2022 Sep;13(3):315-321. doi: 10.30466/vrf.2021.520767.3117. Epub 2022 Sep 15.
species are significant pathogens affecting aquatic species. Around 12 species of can cause a gastrointestinal illness (gastroenteritis) in humans resulting from eating contaminated food such as raw or undercooked shellfish. The indiscriminate use of antibiotics accelerates the development of resistance representing a severe challenge for controlling outbreaks. In this study, the antibiotic resistance profile and the prevalence of pathogenic species of apparently healthy and diseased fishes isolated from different types of fish in Kafr EL-Sheikh Governorate in Egypt during 2018 were determined. Samples obtained from fishes were inoculated onto a -selective medium (TCBS) and phenotypically identified using the biochemical characteristics and representative cultures were checked by PCR to confirm the identified isolates. In the present study, (16.00%) was the predominant species followed by (7.33%) and (5.33%). The tested isolates were resistant to ampicillin (80.00%) and sensitive to ciprofloxacin and norfloxacin (100%). A total number of 15 isolates (five five , and five ) were screened for five housekeeping genes and pathogenic virulence markers by PCR. Results showed that 100% of the isolates carried the gene and 60.00% carried the gene. In , 100% of the isolates carried the gene 0.00% carry the gene; and 80.00% of isolates carried the gene. The results showed that many isolates in this study had virulence characteristics that might correspond with the potential of infections and diseases.
某些物种是影响水生物种的重要病原体。大约12种此类物种可导致人类因食用受污染食物（如生的或未煮熟的贝类）而患上胃肠道疾病（肠胃炎）。抗生素的滥用加速了耐药性的发展，这对控制此类疫情构成了严峻挑战。在本研究中，测定了2018年从埃及卡夫尔谢赫省不同类型鱼类中分离出的看似健康和患病鱼类的抗生素耐药谱以及致病物种的流行情况。从鱼类中获取的样本接种到一种选择性培养基（TCBS）上，并通过生化特征进行表型鉴定，代表性培养物通过PCR进行检查以确认鉴定出的分离株。在本研究中，[具体物种1]（16.00%）是优势物种，其次是[具体物种2]（7.33%）和[具体物种3]（5.33%）。测试的分离株对氨苄青霉素耐药（80.00%），对环丙沙星和诺氟沙星敏感（100%）。通过PCR对总共15株分离株（5株[具体物种1]、5株[具体物种2]和5株[具体物种3]）进行了5个管家基因和致病毒力标记物的筛选。结果显示，100%的[具体物种1]分离株携带[基因1]，60.00%携带[基因2]。在[具体物种2]中，100%的分离株携带[基因3]，0.00%携带[基因4]；80.00%的[具体物种3]分离株携带[基因5]。结果表明，本研究中的许多分离株具有可能与感染和疾病潜力相对应的毒力特征。
