Laganà Pasqualina, Caruso Gabriella, Minutoli Eleonora, Zaccone Renata, Santi Delia
Department of Hygiene, Preventive Medicine and Public Health R. De Blasi, University of Messina, Messina, Italy.
New Microbiol. 2011 Jan;34(1):53-63. Epub 2011 Jan 15.
The antibiotic resistance patterns of aetiological agents responsible for vibriosis and pasteurellosis were studied to contribute to control the spread of these two bacterial diseases in Mediterranean fish farming. Strains of Photobacterium damsela ssp. piscicida, Vibrio fluvialis, Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio metschnikovii, isolated from Italian aquaculture (fish, shellfish and crustaceans) sites, were assayed for their susceptibility to some antibacterial agents currently used in farming practices. Kirby Bauer and Minimum Inhibitory Concentration (M.I.C.) tests were performed. The bacterial strains showed resistance to ampicillin, carbenicillin, kanamycin, cefalothin, while they were sensitive to chloramphenicol, nitrofurantoin and tobramycin; the sulfadiazine-trimethoprim association was completely ineffective. Conversely, flumequine showed the lowest M.I.C. value (0.97 �g mL-1), suggesting its marked antibiotic effect. Considering that quinolone resistance can be transmitted only by selection of mutations and not by other genetic mechanisms, this study stresses the importance of a more responsible use of this antibacterial drug in aquaculture.
为有助于控制这两种细菌性疾病在地中海鱼类养殖中的传播，对引起弧菌病和巴斯德氏菌病的病原体的抗生素耐药模式进行了研究。从意大利水产养殖（鱼类、贝类和甲壳类动物）场所分离出的美人鱼发光杆菌杀鱼亚种、河流弧菌、溶藻弧菌、副溶血性弧菌、梅氏弧菌菌株，被检测了它们对目前养殖实践中使用的一些抗菌剂的敏感性。进行了 Kirby Bauer 试验和最低抑菌浓度（M.I.C.）试验。这些细菌菌株对氨苄青霉素、羧苄青霉素、卡那霉素、头孢噻吩耐药，而对氯霉素、呋喃妥因和妥布霉素敏感；磺胺嘧啶 - 甲氧苄啶组合完全无效。相反，氟甲喹显示出最低的 M.I.C. 值（0.97 μg mL-1），表明其显著的抗生素作用。鉴于喹诺酮耐药性只能通过突变选择而不是其他遗传机制传播，本研究强调了在水产养殖中更合理使用这种抗菌药物的重要性。
