Papadakis G, Friedt J M, Eck M, Rabus D, Jobst G, Gizeli E
Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str, 70013, Heraklion, Greece.
FEMTO-ST Time & Frequency/SENSeOR, 26 rue de l'Epitaphe, 25000, Besançon, France.
Biomed Microdevices. 2017 Sep;19(3):16. doi: 10.1007/s10544-017-0159-2.
The development of integrated platforms incorporating an acoustic device as the detection element requires addressing simultaneously several challenges of technological and scientific nature. The present work was focused on the design of a microfluidic module, which, combined with a dual or array type Love wave acoustic chip could be applied to biomedical applications and molecular diagnostics. Based on a systematic study we optimized the mechanics of the flow cell attachment and the sealing material so that fluidic interfacing/encapsulation would impose minimal losses to the acoustic wave. We have also investigated combinations of operating frequencies with waveguide materials and thicknesses for maximum sensitivity during the detection of protein and DNA biomarkers. Within our investigations neutravidin was used as a model protein biomarker and unpurified PCR amplified Salmonella DNA as the model genetic target. Our results clearly indicate the need for experimental verification of the optimum engineering and analytical parameters, in order to develop commercially viable systems for integrated analysis. The good reproducibility of the signal together with the ability of the array biochip to detect multiple samples hold promise for the future use of the integrated system in a Lab-on-a-Chip platform for application to molecular diagnostics.
开发包含声学设备作为检测元件的集成平台需要同时应对若干技术和科学性质的挑战。目前的工作重点是设计一种微流体模块，该模块与双型或阵列型乐甫波声学芯片相结合，可应用于生物医学应用和分子诊断。基于系统研究，我们优化了流通池连接和密封材料的力学性能，以使流体接口/封装对声波造成的损失最小。我们还研究了工作频率与波导材料和厚度的组合，以在检测蛋白质和DNA生物标志物时实现最大灵敏度。在我们的研究中，中性抗生物素蛋白用作模型蛋白质生物标志物，未纯化的PCR扩增沙门氏菌DNA用作模型遗传靶标。我们的结果清楚地表明，需要对最佳工程和分析参数进行实验验证，以便开发出具有商业可行性的集成分析系统。信号的良好重现性以及阵列生物芯片检测多个样品的能力为该集成系统未来在用于分子诊断的芯片实验室平台中的应用带来了希望。
