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用于同时进行表面等离子体共振和表面声波测量的混合传感器设备。

Hybrid Sensor Device for Simultaneous Surface Plasmon Resonance and Surface Acoustic Wave Measurements.

机构信息

Institute of Molecular Biology & Biotechnology, FO.R.T.H, Vassilika Vouton, 70013 Heraklion, Greece.

Department of Biology, University of Crete, Vassilika Vouton, 71409 Heraklion, Greece.

出版信息

Sensors (Basel). 2020 Oct 29;20(21):6177. doi: 10.3390/s20216177.

DOI:10.3390/s20216177
PMID:33138312
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7662402/
Abstract

Surface plasmon resonance (SPR) and Love wave (LW) surface acoustic wave (SAW) sensors have been established as reliable biosensing technologies for label-free, real-time monitoring of biomolecular interactions. This work reports the development of a combined SPR/LW-SAW platform to facilitate simultaneous optical and acoustic measurements for the investigation of biomolecules binding on a single surface. The system's output provides recordings of two acoustic parameters, phase and amplitude of a Love wave, synchronized with SPR readings. We present the design and manufacturing of a novel experimental set-up employing, in addition to the SPR/LW-SAW device, a 3D-printed plastic holder combined with a PDMS microfluidic cell so that the platform can be used in a flow-through mode. The system was evaluated in a systematic study of the optical and acoustic responses for different surface perturbations, i.e., rigid mass loading (Au deposition), pure viscous loading (glycerol and sucrose solutions) and protein adsorption (BSA). Our results provide the theoretical and experimental basis for future application of the combined system to other biochemical and biophysical studies.

摘要

表面等离子体共振 (SPR) 和乐甫波 (LW) 声表面波 (SAW) 传感器已被确立为用于无标记、实时监测生物分子相互作用的可靠生物传感技术。本工作报道了一种组合 SPR/LW-SAW 平台的开发，以促进光学和声学测量的同时进行，从而研究单个表面上的生物分子结合。该系统的输出提供了与 SPR 读数同步的两种声学参数（乐甫波的相位和幅度）的记录。我们介绍了一种新颖的实验装置的设计和制造，该装置除了 SPR/LW-SAW 器件外，还采用了 3D 打印塑料支架和 PDMS 微流控池，以便平台可以在直通模式下使用。该系统在对不同表面扰动（即，刚性质量加载（金沉积）、纯粘性加载（甘油和蔗糖溶液）和蛋白质吸附（BSA））的光学和声学响应进行了系统研究。我们的结果为未来将组合系统应用于其他生化和生物物理研究提供了理论和实验基础。

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