Neoantimycins A and B, Two Unusual Benzamido Nine-Membered Dilactones from Marine-Derived Streptomyces antibioticus H12-15.

An actinomycete strain (H12-15) isolated from a sea sediment in a mangrove district was identified as on the basis of 16S rDNA gene sequence analysis as well as the investigation of its morphological, physiological, and biochemical characteristics. Two novel benzamido nonacyclic dilactones, namely neoantimycins A () and B (), together with the known antimycins A (,), A (), and A₉ (), were isolated from the culture broth of this strain. Compounds and are the first natural modified ATNs with an unusual benzamide unit. The structures of these new compounds, including their absolute configuration, were established on the basis of HRMS, NMR spectroscopic data, and quantum chemical ECD calculations. Their cytotoxicities against human breast adenocarcinoma cell line MCF-7, the human glioblastoma cell line SF-268, and the human lung cancer cell line NCI-H460 were also tested. All compounds exhibited mild cytotoxic activity. However, Compounds and showed no activity against at the test concentration of 1 mg/mL via paper disc diffusion, while the known antimycins showed obvious antifungal activity.